A naked-eye detection of cholesterol using enzyme cascade reactions on chitosan beads
September 15-19, 2019
Cholesterol is an important biomarker that is involved in the implication of many diseases including coronary heart disease, hypertension, arteriosclerosis and etc. Faster assessment of the cholesterol level in the body may help the users to monitor their health status and allow faster action for the appropriate treatments. A hydrogel based enzyme cascade system using multiple enzymes was developed to detect the cholesterol levels with a naked-eye. Cholesterol esterase, cholesterol oxidase, and horseradish peroxidase were immobilized onto chitosan beads through a glutaraldehyde cross-linkage. The cholesterol level was monitored through the oxidation of ABTS in the assay system. The optimum temperature of the immobilized chitosan beads was 30ºC and the beads retained its activity up to 9 successive operations. We found that glycerol coated immobilized chitosan beads to be more stable in retaining its structure and activity at room temperature over time. Because enzyme has great specificity against its substrate molecules, we believe that the chitosan beads provide an excellent platform for the enzyme cascade system that may be applied in detecting the small molecule biomarkers.
Hyunbeom Lee, "A naked-eye detection of cholesterol using enzyme cascade reactions on chitosan beads" in "Enzyme Engineering XXV", Huimin Zhao, University of Illinois at Urbana-Champaign, USA John Wong, Pfizer, USA Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/enzyme_xxv/65