Enzyme evolution in synthetic biology: A key role from proof-of-concept towards precision function
September 15-19, 2019
Enzyme evolution plays an important role in generating the novel catalytic functions of the enzymes for the desired reactions and thus provides a promising route for bio-manufactures. Here, we give examples for discovering and optimizing the promiscuous enzymes and exploring their uses in reprogramed biosynthetic pathways of Ginsenoside Rh2 and 2-Amino-1,3-propanediol. Through engineering the aminotransferases and the glycosyltransferase in vitro based on their catalytic mechanisms, we successfully repurposed the biosynthetic pathways for high production outcomes in vivo in hosts including Saccharomyces cerevisiae and Escherichia coli. These works demonstrate that enzyme evolution combined with the metabolic engineering is an efficient approach for environmentally friendly productions of pharmaceuticals and chemicals.
Yan Feng, "Enzyme evolution in synthetic biology: A key role from proof-of-concept towards precision function" in "Enzyme Engineering XXV", Huimin Zhao, University of Illinois at Urbana-Champaign, USA John Wong, Pfizer, USA Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/enzyme_xxv/93