Bioprocess characterization at the micro-scale: Optical sensor integration in a novel capillary-wave micro-bioreactor
March 20 – 23, 2022
Due to the high demand of new biopharmaceuticals and bioproducts, the development of new cultivation platforms for high-throughput screenings, cell-based assays and bioprocess development is of high interest. Therefore, micro-bioreactors (MBRs) are a promising alternative to conventional cultivation platforms like shake flasks due to their minimal volume, sensor integration and high ability for automatization and parallelization. Especially, MBRs with a volume below 10 µL can reduce the amount of needed testing substances for cell-based assays, which is advantageous mostly for testing new biopharmaceuticals with limited availability. However, characterization of a cell culture in the lower micro-liter scale is challenging due to the limited space and the insufficient volume for sampling and offline analysis. Optical sensors are one suitable possibility to close this gap. Therefore, a novel capillary-wave micro-bioreactor (cwMBR) with a working volume of 7 µL and optical sensors for biomass, glucose, oxygen, pH and fluorescence intensity measurement was developed.
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Kevin Viebrock, Lasse Frey, Dominik Rabl, Detlev Rasch, Sven Meinen, Andreas Dietzel, Torsten Mayr, and Rainer Krull, "Bioprocess characterization at the micro-scale: Optical sensor integration in a novel capillary-wave micro-bioreactor" in "Single-Use Technologies V: Building The Future", Magali Barbaroux, Sartorius, France; Martina Micheletti, University College London, UK Eds, ECI Symposium Series, (2022). https://dc.engconfintl.org/sut_v/28