Conference Dates

March 20 – 23, 2022


The current shorts in single use (SU) supply chains show how dependent both industry and academia are from only a few vendors worldwide. This is severely hindering fundamental research and process development for the pandemic response. With 3D printing technology we can manufacture SU equipment on demand and on site. In this study we investigated different commercially available low-cost materials and their compatibility for cell culture. We identified poly lactic acid (PLA) as perfect candidate for 3D printed parts for cell culture applications.

The worldwide supply chain issues for SU shaking flasks and reactors gave us the incentive to develop 3D printed counterparts to maintain our HEK293 cell culture. The shake flasks were designed in Autodesk inventor 3D CAD. The materials tested represent the market of different 3D printing technologies and materials, ranging from UV-polymerizing resin printers to thermoplastic printers. We included different manufacturers, plant derived and water washable resins as well as medical Class IIa resins. Whereas resin printed shaking flasks needed washing, curing and sterilization using isopropyl alcohol, the thermoplastic flasks were directly autoclaved. The different materials were tested with HEK293 cells under standard conditions. Cell growth and viability were monitored daily.

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