PURIFICATION PROCESS DEVELOPMENT OF PROTEIN SUBUNIT BASED VACCINE CANDIDATES PRODUCED USING RECOMBINANT E. COLI EXPRESSION SYSTEM (Part - I)
June 6-11, 2010
Proteins are key components of prophylactic vaccines against infectious diseases. Protein subunit based vaccine is an attractive alternative to the traditional detoxified bacterial or inactivated viral vaccine approach due to its highly purified and well characterized product nature. Purification of protein antigens to achieve consistent product purity and quality is an integral part of the protein subunit vaccine product development process. Expression levels of the recombinant proteins in bacterial expression system may be extremely high following rapid technology advancement. The challenges and approaches used to develop purification processes for novel protein vaccine candidates expressed at g/L level will be discussed.
Davinder Chawla and Yan-Ping Yang, "PURIFICATION PROCESS DEVELOPMENT OF PROTEIN SUBUNIT BASED VACCINE CANDIDATES PRODUCED USING RECOMBINANT E. COLI EXPRESSION SYSTEM (Part - I)" in "Vaccine Technology III", John G. Auniņš,Merck, USA; Barry C. Buckland, BiologicB, USA; Kathrin U. Jansen, Pfizer, USA; Paula Marques Alves, IBET, Portugal Eds, ECI Symposium Series, (2010). https://dc.engconfintl.org/vaccine_iii/26