June 12-17, 2016 - Grande Real Santa Eulalia Hotel Albufeira, Portugal

Editors:   Laura Palomares, UNAM, Mexico
  Manon Cox, Protein Sciences Corporation, USA
  Tarit Mukhopadhyay, University College London, UK
  Nathalie Garçon, BIOASTER Technology Research Institute, FR

The articles for these proceedings are not peer-reviewed.

Please click on the PDF icon for the presentation and the title for the abstract.



Conference Program (Vaccine Technology VI), Laura A. Palomares, Tarit Mukhopadhyay, Manon Cox, and Nathalie Garçon (Article)


Vaccines: Reaching for higher branches after the low hanging fruit has been picked, Michael G. Kurilla (Abstract and Presentation)

A universal influenza virus vaccine candidate confers protection against pandemic H1N1 infection in ferrets, Raffael Nachbagauer, Talia Atlas, Angela Choi, Paul Bunduc, Adolfo García-Sastre, Peter Palese, Randy A. Albrecht, and Florian Krammer (Abstract)

Single-cell analysis of Influenza A virus-infected cells for the optimization of cell culture-based vaccine production, Sascha Young Kupke, Frank Stefan Heldt, Timo Frensing, and Udo Reichl (Abstract)


Development of Typhax, a Salmonella Typhi Vi polysaccharide protein capsular matrix vaccine, Kevin P. Killeen (Abstract and Presentation)

Quantitative, molecular-level analysis of the serum antibody repertoire reveals unanticipated features of the response to seasonal influenza vaccination, Jiwon Lee, Daniel R. Boutz, M. Gordon Joyce, Christopher Vollmers, Kwanyee Leung, Andrew P. Horton, Brandon J. DeKosky, Chang-Han Lee, Jason J. Lavinder, Kam Hon Hoi, Yaroslav Tsybovsky, Wing-Pui Kong, Daechan Park, Veronika Chromikova, Ted M. Ross, Andrew D. Ellington, Edward M. Marcotte, John R. Mascola, Florian Krammer, Gregory C. Ippolito, Stephen R. Quake, Peter D. Kwong, and George Georgiou (Abstract)

Upstream and downstream process development of a Vero cell-based yellow fever vaccine, Leda R. Castilho, Tania Pato Cunha, Diogo A. Mattos, Marta Cristina O. Souza, Marlon V. Silva, Elena C. C. S. Campos, Luciane P. Gaspar, and Marcos S. Freire (Abstract)

Fast-track lentiviral vector upstream process development: Leveraging high- throughput process monitoring, single-use bioreactor scalability, Nicolas Sève (Abstract)

Hollow fiber-based high-cell-density and two-stage bioreactor continuous cultivation: Options and limits towards process intensification for virus production, Yvonne Genzel, Daniel Vázquez, Felipe Tapia, Ingo Jordan, and Udo Reichl (Abstract)

A live attenuated RSV vaccine, process development studies, Yvonne E. Thomassen, Joyce van der Welle, Sven van der Kooi, Rimko ten Have, and Pauline Verdijk (Abstract)

Genetic engineering of vaccine manufacturing cell lines enhances poliovirus and enterovirus 71 production, Jon Karpilow, Ralph Tripp, Mark Tompkins, Weilin Wu, Steve Oberste, and Sabine van der Sanden (Abstract)

Adjuvants in preclinical and clinical development: The do and don’t, Nathalie Garçon (Abstract)


Combining DOE with an empirical approach to improve vaccine formulation development, Jill Livengood, Tim Powell, and Melissa Bushey (Abstract and Presentation)

Development of a thermostable ID93 + GLA-SE vaccine using a design of experiments (DOE) approach, Ryan M. Kramer (Abstract)


Controlled, pulsatile release of thermostabilized inactivated polio vaccine from PLGA-based microspheres, Stephany Y. Tzeng, Rohiverth Guarecuco, Kevin J. McHugh, Evan M. Rosenberg, Yingying Zeng, Robert Langer, and Ana Jaklenec (Abstract and Presentation)

Current technologies for advancing HIV vaccines, Vadim Tsvetnitsky (Abstract)

Applications of DNA vaccine technology towards difficult immune targets, David B. Weiner (Abstract)

Advancing the mRNA therapeutics platforms for vaccines, Hari Pujar (Abstract)

Vaccine based immunotherapy regimen (VBIR) for the treatment of prostate cancer, Helen Cho (Abstract)

Development of immunotherapeutic immunizations for virus infections and cancer, Ian Frazer (Abstract)


Session V: Getting Vaccines to the Market: Case studies, Rebecca Sheets, Grimalkin Partners, and Danilo Casimiro (Abstract and Presentation)

RSV vaccines for the Young and the old, Albert Price (Abstract)


Development, manufacturing, and supply of MSD’s Ebola vaccine, Jeffrey T. Blue (Abstract and Presentation)

Third generation vaccine for world eradication of poliomyelitis, Emilie Rodrigues, Aart van t Oever, Gerco van Eikenhorst, Umut Demirtas, Andrew Macadam, Yvonne Thomassen, and Wilfried Bakker (Abstract)


Improving global human health through norovirus virus-like particle manufacturing, Scot Shepard (Abstract and Presentation)

How is vaccine R&D pipeline strategy going to evolve for pharm industry…?, Johan Van Hoof (Abstract)

Challenges in the development and scale-up of a purification process for an attenuated live virus vaccine candidate, Matthew Woodling, Sianny Christanti, Adam Kristopeit, Marc Wenger, and Joseph Joyce (Abstract)

Insect cells platforms for fast production of Pseudo-Typed VLPs for drug and vaccine development, António Roldão, João Vidigal, Daniela Sequeira, Bárbara Fernandes, Mafalda M. Dias, Marco Patrone, Manuel JT Carrondo, Ana P. Teixeira, and Paula M. Alves (Abstract)


Determining whether adsorption state is a critical attribute in aluminum adjuvanted vaccines, Garry Morefield (Abstract and Presentation)

Analytical characterization of human Cytomegalovirus vaccine and vaccine induced humoral responses, Sha Ha (Abstract)

Multi-tasking an inactivated influenza vaccine to provide rapid innate immune- system mediated protection and subsequent long-term adaptive immunity against influenza and secondary pneumococcal infections, Brendon Y. Chua, Chinn Yi Wong, Edin J. Mifsud, Kathryn M. Edenborough, Toshiki Sekiya, Amabel CL Tan, Francesca Mercuri, Steve Rockman, Weisan Chen, Stephen J. Turner, Peter C. Doherty, Anne Kelso, Lorena E. Brown, and David C. Jackson (Abstract)

Correlations of antibody response phenotype to genotype revealed by molecular amplification fingerprinting, Sai T. Reddy, Tarik A. Khan, Simon Friedensohn, and Arthur R. Gorter de Vries (Abstract)

Immune engineering enhances H7N9 vaccine immunogenicity by regulatory T cell epitope deletion in hemagglutinin, Annie De Groot, Lenny Moise, Rui Liu, Bradford C. Lefoley, Rayleigh Chan, Manabu Ato, Yoshimasa Takahashi, Arnone Nithichanon, Ted M. Ross, and William Martin (Abstract)


Vaccination as a tool to reduce antimicrobial resistance worldwide, Bernard VALLAT and Elisabeth Erlacher (Abstract and Presentation)

Structural-based designed modular capsomere comprising HA1 as low-cost poultry influenza vaccine, Jarurin Waneesorn, Anton PJ Middelberg, and Linda HL Lua (Abstract)

Development of a vaccine based on recombinant subunit proteins to protect humans and animals against filovirus disease, Axel T. Lehrer, Teri-Ann S. Wong, and Michael M. Lieberman (Abstract)


How to deliver new vaccines under very short timelines: The ZAPI project, Jean-Christophe AUDONNET (Abstract and Presentation)

From virus discovery to intervention, Ab Osterhaus (Abstract)

Systems biology and single cell approaches to study human immune responses to vaccination, John S. Tsang (Abstract)

Structure-based Vaccine Design: Lessons from RSV F, Jason McLellan (Abstract)

Induction of antigen-specific immune tolerance with synthetic nanoparticle vaccines, Takashi Kei Kishimoto and Petr Ilyinskii (Abstract)

Universal and in-process analytical tool for Influenza quantification using a label-free technology, Sofia Carvalho, Mafalda Moleirinho, David Wheatley, John Welsh, René Gantier, Paula Alves, Cristina Peixoto, and Manuel Carrondo (Abstract)

Applications of high-throughput single B-cell sequencing to accelerate rational vaccine design, Brandon DeKosky, Erica Normandin, Cheng Cheng, Hongying Duan, Xuejun Chen, Maryam Mukhamedova, Sarah Andrews, Amy Ransier, Sam Darko, Hugh Welles, Mario Roederer, Peter Kwong, Danny Douek, Adrian McDermott, Nancy Sullivan, and John Mascola (Abstract)


Plant-based technologies to enable rapid response to Ebola outbreak, Jerzy Karczewski and Vidadi Yusibov (Abstract and Presentation)

Causes, challenges and solutions for developing vaccines for low-income settings, Katey Owen (Abstract)

Influenza vaccine production using cell culture with microcarriers, Michael McGlothlen and Lori Mullen (Abstract)

Evaluation and scale-up of single-use bioreactors for the production and harvesting of a hepatitis C vaccine candidate, Alex Xenopoulos, Lénaïg Savary, Ricardo Silva, João Clemente, Rute Castro, Sofia B. Carvalho, Mafalda Moleirinho, Paula Alves, Manuel Carrondo, and Cristina Peixoto (Abstract)

Computational fluid dynamics modeling for HPV fermentation bioreactors, Tracie Spangler and Matthew Flamm (Abstract)

Development of a stabilized trimer pre-fusion RSV F recombinant viral glycoprotein vaccine, Richard M. Schwartz, Althaf I. Hussain, Mingzhong Chen, Pefieng Chen, Mridul Ghosh, Jonathan W. Cooper, Sarah E. O’Connell, and Lisa A. Kueltzo (Abstract)

Optimization of sulfated cellulose membrane adsorbers for the purification of influenza virus, A. Raquel Fortuna, Sebastian van Teeffelen, Florian Taft, Louis Villain, Michael M. Wolff, and Udo Reichl (Abstract)

Purification of cell culture-derived influenza virus via continuous chromatography, Laura M. Fischer, Sibel Ilhan, Michael W. Wolff, and Udo Reichl (Abstract)

Optimization and scale-up of cell culture and purification processes for production of an adenovirus-vectored tuberculosis vaccine candidate, Chun Fang Shen, Danielle Jacob, Tao Zhu, Alice Bernier, Zhongqi Shao, Xuefeng Yu, Mehul Patel, Stephane Lanthier, Sven Ansorge, and Amine Kamen (Abstract)

Pan-HA antibodies for influenza detection and quantification, Aziza P. Manceur; Anne Marcil; Wei Zou; Wangxue Chen; Christine Gadoury; Emma Petiot; Amine A. Kamen; Xuguang (Sean) Li,; Bozena Jaentschke; and Sven Ansorge (Abstract)

High titer production of HIV-1 virus-like particles by CAP-T cells, Sonia Gutiérrez-Granados, Laura Cervera, Mercedes Segura, Jens Wölfel, and Francesc Gòdia (Abstract)


Characterization of HA and NA-containing VLPs produced in suspension cultures of HEK 293 cells, Amine Kamen, Alina Venereo-Sanchez, Rénald Gilbert, Aziza Manceur, Parminder Chahal, Sven Ansorge, and Wangxue Chen (Abstract and Presentation)

Novel avian DuckCeltTM-T17 cell line for production of viral vaccines : application to influenza viruses production, Emma Petiot, Anais Proust, Aurélien Traversier, Manuel Rosa-Calatrava, and Jean-Marc Balloul (Abstract)

Pseudo-affinity purification and formulation of a cell-culture derived whole influenza virus vaccine using magnetic sulfated cellulose particles, Michael M. Pieler, Sarah Frentzel, Dunja Bruder, Michael W. Wolff, and Udo Reichl (Abstract)

Trumenba: A case study for development of a drug substance manufacturing process through commercialization, Khurram Sunasara (Abstract)

A stabilized subunit vaccine for ebola virus, Keith Chappell, Daniel Watterson, David Muller, Alexandra Depelsenaire, Andrew Young, Glenn Marsh, Mark Kendal, and Paul Young (Abstract)

Improved seed train strategy applied to PER.C6® cells for manufacturing readiness in vaccines production, Piergiuseppe Nestola, Sabine Kuratli, and Claudio Thomasin (Abstract)

History and development of a liquid formulation for adenoviral vaccines, Lara Babich, Els van Deventer, and Janik Adriaansen (Abstract)

Tetraspanins displayed in retrovirus-derived virus-like particles and their impact in vaccine development, Hugo Soares, Patricia Gomes-Alves, Paula Alves, Manuel Carrondo, and Ana Sofia Coroadinha (Abstract)

A modular approach for efficient production of multi-HA Influenza VLP-based vaccines, António Roldão, Daniela Sequeira, Ricardo Correia, Manuel JT Carrondo, Ana P. Teixeira, and Paula M. Alves (Abstract)

Improving downstream processing of enveloped virus-like particles with multi-column chromatography, Ricardo Silva, Sofia B. Carvalho, Alex Xenopoulos, Paula Alves, Cristina Peixoto, and Manuel Carrondo (Abstract)

A click chemistry strategy to specifically monitor and improve purification of Influenza virus-like particles, Sofia Carvalho, João Freire, Mafalda Moleirinho, Francisca Monteiro, Diana Gaspar, Miguel Castanho, Paula Alves, Gonçalo Bernardes, Cristina Peixoto, and Manuel Carrondo (Abstract)

Enveloped virus-like particles purification using an all-filtration technology platform, Sofia Carvalho, Ricardo Silva, Bárbara Cunha, Ana Sofia Moreira, Mafalda Moleirinho, Alex Xenopoulos, Paula Alves, Cristina Peixoto, and Manuel Carrondo (Abstract)

The papaya mosaic virus (PapMV) nanoparticles; a promising tool in vaccine development., Denis Leclerc, Marilène Bolduc, Marie-Eve laliberté, Alain Lamarre, and Pierre Savard (Abstract)

Novel pulsatile-release microparticles for single-injection vaccination, Kevin J. McHugh, Thanh D. Nguyen, David Yang, Allison R. Linehan, Jennifer Lu, Stephany Y. Tzeng, Adam M. Behrens, Robert S. Langer, and Ana Jaklenec (Abstract)

Structurally confined influenza subunit vaccines in the prefusion conformation elicit a potent neutralizing antibody response, Daniel Watterson, Keith J. Chappell, Christopher McMillan, Stacey Cheung, and Paul R. Young (Abstract)

Vaccination with recombinant neuraminidase protects against influenza virus infection in mice, Teddy John Wohlbold, Veronika Chromikova, Philip Meade, Fatima Amanat, Ariana Hirsh, Raffael Nachbagauer, Gene S. Tan, Peter Palese, and Florian Krammer (Abstract)

Residual DNA analysis in influenza vaccine processing, Camilla Estmer Nilsson, Hanna Kraft, Christine Sund Lundström, Anna Mattson, and Elisabeth Wallby (Abstract)

Study of rabies VLPs expression in BHK-21 cell line for vaccine applications, Claudio Prieto, Ernesto Garay, Diego Fontana, Marina Etcheverrigaray, and Ricardo Kratje (Abstract)

Expression of rabies VLPs in adherence and suspension conditions: a flexible platform for rabies vaccine production, Diego Fontana, Ernesto Garay, Claudio Priet, and Ricardo Kratje (Abstract)

Process economy effects of modernizations in vaccine purification, Mia Bennemo (Abstract)

Propagation of influenza and MVA virus in cascades of continuous stirred tank bioreactors: challenging the "Von Magnus effect", Felipe Tapia, Yvonne Genzel, Ingo Jordan, Volker Sandig, and Udo Reichl (Abstract)

Intensification of MVA and influenza virus production through high-cell-density cultivation approaches, Daniel Vázquez, Yvonne Genzel, Michael M. Pieler, Ingo Jordan, Volker Sandig, and Udo Reichl (Abstract)


Production of a Nanoplasmid™ with a large gene insert using the HyperGRO™ fermentation process, Aaron Carnes, Neha Tiwari, Jill Beilowitz, Carlos Sampson, Dorothy Peterson, and Jim Williams (Abstract and Presentation)

Virus-like particles adsorption in anion exchange chromatography media, Patricia Pereira Aguilar, Tobias Schneider, and Alois Jungbauer (Abstract)

Ready to use gamma irradiated microcarriers for virus production in single use bioreactor systems, Gustaf Ahlén and Eva Blanck (Abstract)

Development of a versatile vaccination platform based on papaya mosaic virus (PapMV) nanoparticles, Ariane Thérien, Damien Carignan, Gervais Rioux, and Denis Leclerc (Abstract)

Evaluation of producer cell lines for yellow fever virus production in up to 1 L bioreactor scale, Alexander Nikolay, Katharina Hermann, Yvonne Genzel, and Udo Reichl (Abstract)

Propagation of Brazilian Zika virus strains in static, microcarrier-based and suspension cultures using BHK and Vero cells, Alexander Nikolay, Leda Castilho, Amilcar Tanuri, Udo Reichl, and Yvonne Genzel (Abstract)

Electron microscopy as an emerging analytical tool for characterizing vaccines, Anette Schneemann, Joyce J. Sung, Jeffrey A. Speir, Sean K. Mulligan, Kat On, Joel Quispe, Bridget Carragher, and Clinton S. Potter (Abstract)

Optimizing scale-up of Vero cells cultured on microcarriers in serum-free medium for vaccine production, Anna-Barbara Hachmann, Andrew M. Campbell, and Stephen F. Gorfien (Abstract)

Custom open polyethersulfone ultrafiltration membranes for vaccines, Clifton Ngan (Abstract)

Highly cross-conserved burkholderia T cell epitopes generate effector T cell responses in vitro, Annie De Groot, Rui Liu, Lenny Moise, Ryan Tassone, Mohab Ali, Matthew Ardito, Frances Terry, and William Martin (Abstract)

Predicting tolerance in vaccine antigens: Application to influenza, HCV and HIV, Annie De Groot, Rui Liu, Lenny Moise, Ryan Tassone, Frances Terry, and William Martin (Abstract)

In vivo validation of predicted and conserved T cell epitopes in a swine influenza model, Andres H. Gutiérrez, Crystal Loving, Susan L. Brockmeier, Holly R. Hughes, Leonard Moise, Frances E. Terry, William D. Martin, and Anne S. De Groot (Abstract)

Development of insect cell platforms for fast production of pseudo-typed VLPs for drug and vaccine development, João Vidigal, Bárbara Fernandes, Mafalda M. Dias, Manuel JT Carrondo, Paula M. Alves, Ana P. Teixeira, and Marco Patrone (Abstract)

Safe and green, the hyperbar inactivation, Fabien Lux (Abstract)

Influenza A virus propagation in MDCK: Intracellular virus replication, virus release and cell-cycle preferential infection analysis, Gallo Ramírez; Frensing T. Reichl; and Kupke, S, Bachmann Fritzsche (Abstract)

VP2 potentiates the proteccion induced by VP6 against the rotavirus infection in a DNA vaccine model, López-Guerrero DV, Gutiérrez-Xicotencatl L, Pedroza A, Chiu L, Plett T, Villegas J, Rosas G, Arias N, López S, Arias C, and Fernández G (Abstract)

Differential antibody response against conformational and linear epitopes of the L1 protein from human papillomavirus types 16/18 is generated in vaccinated woman or with different exposures to the virus, Gutierrez-Xicotencatl L., Pedroza-Saavedra A, Chihu-Amparan L, Maldonado-Gama M, Salazar-Piña DA, Rodríguez-Ocampo AN, Cruz-Valdez A, Carmona-Balandrano E, and Esquivel-Guadarrama F (Abstract)

N-Glycosylation affects humoral immune response of Her1 cancer vaccine, Katia Garcia Duardo, Yanet Cueto Cabañas, Gretchen Bergado Baez, Judith Raymond Pous, Estela Yamilet Rabasa Legón, Adolfo Castillo Vitlloch, and Kathya R. de la Luz Hernández (Abstract)

IL-17A and Streptococcus pneumoniae respiratory infection: Prospects for the development of new immunotherapies, Rial A., Céspedes M.P, Comas V, Marqués J.M, Muñoz N, Iwakura Y, and J.A. Chabalgoity (Abstract)

Transferring methods for vaccine release between the industry, academy and a regulatory agency: Lessons learned, Elizabeth Carrasco-Caballero, Momena Choudhury, Katia Esqueda, Manon Cox, Elena Semenova, Manuel Cardoso, Sergio Valentinotti, A. Ruth Pastor, and Laura A. Palomares (Abstract)

Unmasking stem-specific broadly neutralizing epitopes by abolishing N-linked glycosylation sites for vaccine design, Suh-Chin Wu, Wen-Chun Liu, Yun-Ju Huang, and Jia-Tsrong Jan (Abstract)

Improved diagnostics and surveillance identify novel reassortant swine influenza A viruses in Chile, Rafael A. Medina, Barbara Brito, Rodrigo Tapia, Marco Saavedra, Karla Tapia, Montserrat Torremorell, and Victor M. Neira (Abstract)

First characterization of immunogenic conjugates of vi negative salmonella typhi O- specific polysaccharides with rEPA protein for vaccine development, Salman M, Ali A., Rahman M, Iqbal M, Haque A, and Cox A. D (Abstract)


Development of a production process for a recombinant protein pneumococcal vaccine, Ana Maria P. Santos, Mariana Miguez, Ana Paula Argondizzo, and Maria Helena Rocha-Leão (Abstract and Presentation)