ECI Digital Archives

Title

Continuous antibody capture step based on Magnetic Beads

Authors

Nils Arnold Brechmann, AdBIOPRO, Competence Centre for Advanced BioProduction by Continuous Processing, SwedenFollow
Joaquin Gomis Fons, AdBIOPRO, Competence Centre for Advanced BioProduction by Continuous Processing, Sweden; Department of Chemical Engineering, Lund University, Lund, Sweden
Kristofer Eriksson, AdBIOPRO, Competence Centre for Advanced BioProduction by Continuous Processing, Sweden; Lab-on-a-Bead AB, Uppsala, Sweden
Sven Oscarsson, Dept. of Organic Chemistry, Stockholm University, Stockholm, Sweden
Atefeh Shokri, AdBIOPRO, Competence Centre for Advanced BioProduction by Continuous Processing, Sweden; CETEG, Dept. of Industrial Biotechnology, CBH School, KTH, Stockholm, Sweden
Bernt Nilsson, AdBIOPRO, Competence Centre for Advanced BioProduction by Continuous Processing, Sweden; Department of Chemical Engineering, Lund University, Lund, Sweden
Véronique Chotteau, AdBIOPRO, Competence Centre for Advanced BioProduction by Continuous Processing, Sweden; CETEG, Dept. of Industrial Biotechnology, CBH School, KTH, Stockholm, Sweden

Conference Dates

October 6-10, 2019

Abstract

The integration of a perfusion culture process with periodic counter chromatography currently draws a lot of attention in industry, with the goal of generating more efficient and flexible processes at reduced footprint. Purification process based on magnetic beads has been used for decades for analytical purposes. It shows interesting potential and begins to gain interest in the biopharmaceutical industry for fed-batch processes. This type of separation has characteristics that can facilitate continuous process integrated to perfusion operation. However integrated mAb capture using magnetic beads has not yet been published.

In this study we have designed novel system and process for the affinity purification of monoclonal antibodies (mAbs) using magnetic beads based on work we performed in a pilot-scale set-up¹. High capacity magnetic protein A agarose beads (LOABeads PrtA) were used with a prototype separation system for this development. In a proof-of-concept, the prototype set-up was tested with supernatant generated from perfusion process. As shown in Figure 1, the integrated process had a rapid adsorption of the mAb within 2 hours. It provided an overall adsorption of 94% and a yield of 87% (Figure 2). The unique use of high capacity magnetic beads together with this novel prototype system for continuous separation showed promising results, which can provide an efficient way for the integration of up-stream and down-stream process.

Please click Additional Files below to see the full abstract.

Recommended Citation

Nils Arnold Brechmann, Joaquin Gomis Fons, Kristofer Eriksson, Sven Oscarsson, Atefeh Shokri, Bernt Nilsson, and Véronique Chotteau, "Continuous antibody capture step based on Magnetic Beads" in "Integrated Continuous Biomanufacturing IV", Veena Warikoo, Roche, USA Alois Jungbauer, BOKU, Austria Jon Coffman, Boehringer Ingelheim, USA Jason Walther, Sanofi, USA Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/biomanufact_iv/2