Title
Chemical identity and mechanisms of action and formation of a cell growth inhibitory leachable compound from disposable polycarbonate plastic vessels
Conference Dates
May 7-10, 2017
Abstract
This presentation reports the chemical identity and mechanisms of action and formation of a single cell growth inhibitory leachable compound from disposable polycarbonate plastic vessels. 18 leachable compounds were separated and enriched from the total extracts of the vessels. Out of these compounds, only one displayed selective inhibition of the growth of a CHO cell line. High resolution MS and NMR suggested that the leachable compound is a dinitrobisphenol A. Chemical synthesis further confirmed that this leachable compound is one of the seven possible dinitrobisphenol A. Cell assays revealed that this leachable compound acted not only as a cell cycle arrest agent without any effect on cell viability, but also as a GPR35 agonist with moderate potency. Examining the manufacturing process of polycarbonate vessels showed that the formation of this leachable compound requires the combination of three critical process parameters, high temperature molding process, gamma-irradiation, and the presence of air. This study highlights that besides the resin materials the manufacturing process also contributes to the formation of cell growth inhibitory leachable compounds.
Recommended Citation
Zara Melkoumian and Ye Fang, "Chemical identity and mechanisms of action and formation of a cell growth inhibitory leachable compound from disposable polycarbonate plastic vessels" in "Single-use Technologies II: Bridging Polymer Science to Biotechnology Applications", kta Mahajan (Genentech, Inc., USA) Gary Lye (University College London, UK) Regine Eibl-Schindler (Zurich University of Applied Science, Switzerland) Eds, ECI Symposium Series, (2017). https://dc.engconfintl.org/biopoly_ii/44