ECI Digital Archives

Title

De-scattering with excitation patterning in temporally-focused microscopy (DEEP- TFM)

Authors

Jong Kang Park, MIT, USAFollow
Dushan N. Wadduwage, Center for Advanced Imaging, Faculty of Arts and Sciences, Harvard University, USA
Peter T. C. So, Department of Biological Engineering, Department of Mechanical Engineering, Laser Biomedical Research Center, Massachusetts Institute of Technology, USA

Conference Dates

June 2-6, 2019

Abstract

Point-scanning two-photon microscopy is used routinely for in vivo, volumetric biological imaging, especially in deep tissues. Despite the excellent penetration depth, a conventional point-scanning two-photon microscopy is slow due to the need for raster scanning and imaging time scales linearly with increasing volume, hampering studies of fast biological dynamics. An attractive alternative to point-scanning geometries is wide-field two-photon microscopy, typically called temporal focusing microscopy (TFM) since optical sectioning is achieved by focusing a beam temporally while maintaining wide-field illumination. However, TFM suffers from scattering in tissue resulting in limited imaging depth.

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Recommended Citation

Jong Kang Park, Dushan N. Wadduwage, and Peter T. C. So, "De-scattering with excitation patterning in temporally-focused microscopy (DEEP- TFM)" in "Advances in Optics for Biotechnology, Medicine and Surgery XVI", Erin Buckley, Emory University/Georgia Institute of Technology, USA Christophe Moser, Polytechnique Fédérale de Lausanne (EPFL), Switzerland Brian Pogue, Dartmouth College, USA David Sampson, University of Western Australia, Australia Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/biotech_med_xvi/17