Conference Dates
May 8-13, 2016
Abstract
Abstract: In biosimilars development, matching the glycosylation pattern of the innovator molecule can be a significant challenge in cell culture development. Here we present a novel downstream method using Fc gamma receptors as chromatography affinity ligands to separate monoclonal antibody glycoforms. Additional data is presented highlighting glycoform potentiation of effector functions such as antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis which can be important mechanisms of action to consider in biosimilars development
Recommended Citation
Austin Boesch, "Separation of IgG glycoforms for biosimilars development using Fc gamma receptors as affinity-based chromatography ligands" in "Cell Culture Engineering XV", Robert Kiss, Genentech Sarah Harcum, Clemson University Jeff Chalmers, Ohio State University Eds, ECI Symposium Series, (2016). https://dc.engconfintl.org/cellculture_xv/241