September 24-28, 2017
Pierre Baudis Congress Center
Toulouse, France

Editors:   Pierre Monsan, Toulouse White Biotechnology, France
  Magali Remaud-Simeon, LISBP-INSA, University of Toulouse, France

The articles for these proceedings are not peer-reviewed.


Engineering biofilm-blocking enzymes, Shereen Asha Murugayah and Monica Gerth (Abstract)

Rapid enzyme stabilization by computationally designed libraries of HMF oxidase, Caterina Martin, Marco W. Fraaije, and Hein Weijma (Abstract)

The pyrroloquinoline-quinone (PQQ)-dependent quinohemoprotein pyranose dehydrogenase from Coprinopsis cinerea (CcPDH), belonging to the AA12 family, drives lytic polysaccharide monooxygenase (LPMO) action, Aniko Varnai, Kiwamu Umezawa, Makoto Yoshida, and Vincent G.H. Eijsink (Abstract)

Substrate-based protein engineering of a flavoprotein oxidase for improved alcohol over-oxidation, Mathias Pickl, Silvia M. Glueck, and Kurt Faber (Abstract)

Using strucutral information for predicting NAD(P)(H) cofactor specificity, while unveiling the responsible molecular determinants, in enzymes with unknown structure, Tiago Resende, Isabel Rocha, and Cláudio Soares (Abstract)

Production of rhamnolipids-producing enzymes of Pseudomonas in E. coli and structural characterization, Qingxin Li (Abstract)

Microbial production of rhamnolipids from isolate pseudomonas sp. —A mono- rhamnolipid producer, Hui Qing Chong and Qingxin Li (Abstract)

Enzymatic esterification of lactones in aqueous buffer, Lucas Hammerer, Verena Resch, and Wolfgang Kroutil (Abstract)

Peptidase-lipase bifunctional enzyme expressed in pichia pastoris, Hamilton Cabral, Ana Claudia Rodrigues de Siqueira, and Rafael Pedezzi (Abstract)

Microbial production of lipopeptides as biosurfactants for varied applications, Jin Chuan Wu, Ong Shufeng Angeline, and Christoph Johann Heinrich Ottenheim (Abstract)

Switching the cofactor specificity of an imine reductase, Bettina M. Nestl and Niels Borlinghaus (Abstract)

Generation of new imine reducing enzymes - expansion of the imine reductase sequence space, Maike Lenz, Silvia Fademrecht, Bernhard Hauer, Bettina Nestl, and Jürgen Pleiss (Abstract)

Broadening the substrate scope of strictosidine synthases by site-directed mutagenesis, Elisabeth Eger, Eva Fischereder, Horst Lechner, Wolfgang Kroutil, Desiree Pressnitz, Mahima Sharma, and Gideon Grogan (Abstract)

Enzymatic synthesis of glucan dendrimer for pharmaceutical applications, Michiyo Yanase, Ryo Kakutani, Takeshi Takaha, Takashi Kuriki, Saori Hokari, Yoshiaki Yuguchi, and Jun-ichi Kadokawa (Abstract)

How the α-substititionof substrate affects the specific activity and stereoselectivity of carbonyl reductase, Xi Chen, Hongliu Zhang, Jinhui Feng, Qiaqing Wu, and Dunming Zhu (Abstract)

Rieske non-heme dioxygenases: Versatile biocatalysts for the generation of vicinal Cis- Diols, Julia M. Halder, Bernhard Hauer, and Bettina M. Nestl (Abstract)

Structure and function of unusual Rieske-type oxygenases from human microbiota involved in carnitine metabolism, Yun-Bin Han, Lan Xu, and Ian A. Wilson (Abstract)

Crystal structure of a novel (R)-selective amine transaminase and approaches to broaden its substrate scope by rational engineering, Aline Telzerow, Martin Schürmann, Helmut Schwab, and Kerstin Steiner (Abstract)

Design of novel enzymed-catalyzed reactions linked to protein sequences for finding enzyme engineering targets, Jasmin Hafner, Homa Mohammadi Peyhani, Noushin Hadadi, and Vassily Hatzimanikatis (Abstract)

Enhancement of activity and thermostability of a Geobacillus endoglucanase via a unique self-truncation process, Mei-huey Wu, Su-May Yu, Chen-wei Li, and Tuan-Hua David Ho (Abstract)

Computational protein design to accelerate the conception of fine-tuned biocatalysts, Sophie Barbe, Simoncini David, Antoine Charpentier, Seydou Traoré, Isabelle André, Clément Viricel, Juan Cortés, David Allouche, and Thomas Schiex (Abstract)

PockeMO - the structure of a robust polycyclic ketone monooxygenase as a scaffold for engineering biocatalysts active on bulky substrates, Maximilian Josef Ludwig Johannes Fürst, Simone Savino, J. Rúben Gómez Castellanos, Stefano Rovida, Andrea Mattevi, Hanna M. Dudek, Marco W. Fraaije, and Cora Gutiérrez de Souza (Abstract)

Engineering the enantioselective reduction of citral isomers in NCR ene reductase, Nico Kreß, Bernhard Hauer, and Johanna Rapp (Abstract)

Functional metagenomic screening approach for discovery of new glycoside phosphorylases, Spencer S. Macdonald and Stephen G. Withers (Abstract)

Site-directed mutagenesis of structural hot spots for enhanced solubility of deoxyxylulose phosphate pathway enzymes, Xixian Chen, Congqiang Z hang, and Heng-phon Too (Abstract)

Metabolic design of Escherichia coli for muconic acid production, Ryosuke Fujiwara, Shuhei Noda, Akihiko Kondo, and Tsutomu Tanaka (Abstract)

Docking and molecular dynamics approach for enzyme selection for α, β-reduction of enoate moiety: Toward renewable production of adipic acid, Jaeho Shin (Abstract)

Microbial platform to synthesize chorismate derivatives via metabolic engineering approach, Shuhei Noda, Akihiko Kondo, and Tomokazu Shirai (Abstract)

An extracellular protein expression system in Escherichia coli implies potential application, Qingsheng Qi, Dongfang Gao, Quanfeng Liang, and Junshu Wang (Abstract)

Engineering DNA polymerases for application in DNB-based sequencing technology, Yue Zheng, Lin Wang, Wenwei Zhang, and Yuliang Dong (Abstract)

Enhancement of lipase selectivity by site directed mutagenesis, Katja Zorn, Uwe T. Bornscheuer, Isabel Oroz-Guinea, and Henrike Brundiek (Abstract)

Metabolic engineering of S. pombe via CRISPR-Cas9 genome editing for lactic acid production from glucose and cellobiose, Tsutomu Tanaka, Aiko Ozaki, Seiya Takayama, and Akihiko Kondo (Abstract)

Identification of keratinolytic function in Chryseobacterium camelliae Dolsongi-HT1 isolated from Green Tea, Eun-Mi Kim, Jun-Seong Park, and Kyeonghwan Hwang (Abstract)

Sortase A-assisted metabolic enzyme ligation in Escherichia coli, Takuya Matsumoto, Tsutomu Tanaka, and Akihiko Kondo (Abstract)

Discovery, characterization and engineering of bacterial thermostable cellulose- degrading enzymes, Marianne S. Jensen, Lasse Fredriksen, Phil B. Pope, Heidi Østby, Benoît Kunath, Gustav Vaaje-Kolstad, Vincent G.H. Eijsink, Bjørn Altermark, Arne O. Smalås, Runar Stokke, and Ida H. Steen (Abstract)

Optimizing the phosphorus cycle in the sugar beet production process by phytase supplement, Wei Long, Anna Joelle Ruff, and Ulrich Schwaneberg (Abstract)

Critical role of metals in biochemical properties of xylose isomerase, Misun Lee, Dick B. Janssen, Henriette J. Rozeboom, Maarten D. Verhoeven, Jack T. Pronk, and Paul de Waal (Abstract)

Development of a novel homogeneous immunoassay using mutant beta-glucuronidase, Jiulong Su, Hiroshi Ueda, and Jinhua Dong (Abstract)

Stereodivergent cyclopropanation of unactivated alkenes with heme proteins, Anders M. Knight, S.B. Jennifer Kan, Russell D. Lewis, Oliver F. Brandenberg, and Frances H. Arnold (Abstract)

Rational enhancement of the enantioselectivity of Candida antarctica lipase B in kinetic resolution of N-(2-ethyl-6-methylphenyl) alanine, Liangyu Zheng, Suoqin Zhang, and Yu Han (Abstract)

Characterization of a glucose-tolerant β-1,4-glucosidase BglC from Cytophaga hutchinsonii, Xuemei Lu and Yuxiang Zhang (Abstract)


Computational redesign of transaminase active site, Elisa Lanfranchi, Hein J. Wijma, Dick B. Janssen, Carlos J. Ramírez, and Madhurya Lutikurti (Abstract and Presentation)

Engineering bacterial nitroreductases for anticancer gene therapy and targeted cell ablation, Abigail V. Sharrock, Elsie Williams, Vic Arcus, Jeff Mumm, and David Ackerley (Abstract)

Simultaneous randomisation of eight key active site residues in E. coli NfsA to generate superior nitroreductases for prodrug activation, Kelsi R. Hall, Katherine Robins, David Ackerley, Michelle Rich, Rory Little, Janine Copp, Abigail Sharrock, Wayne Patrick, and Jeff Mumm (Abstract)

Use of positive selection methods for discovery and improvement of nitroreductase enzymes for cancer gene therapy, Michelle H. Rich, David Ackerley, Jeff Smaill, Adam Patterson, Elsie Williams, and Rory Little (Abstract)

Development of a selection to recover improved DNA ligase enzymes during directed evolution, Katherine J. Robins, Luke Stevenson, David Ackerley, and Wayne Patrick (Abstract)

Engineering the indigoidine-synthesising enzyme BpsA for diverse applications in biotechnology, Alistair S. Brown, Katherine Robins, Jeremy Owen, and David Ackerley (Abstract)

Engineering a lipase for organic cosolvent resistance - How do current directed evolution approaches compete with the potential that nature offers?, Ulrich Markel, Victorine Josiane Frauenkron-Machedjou, Jing Zhao, Mehdi D. Davari, Marco Bocola, Leilei Zhu, Ulrich Schwaneberg, and Karl-Erich Jaeger (Abstract)

Enzymatic synthesis of cyclic imino acids, Ryoma Miyake, Hiroshi Kawabata, Yasumasa Dekishima, and Takanobu Iura (Abstract)

Metabolic engineering of Saccharomyces cerevisiae to harness natures valuable compounds, Christian Nyffenegger, Ângela Carvalho, Klavs Riishede Hansen, Esben Halkjær Hansen, Jørgen Hansen, Jean Amick, and Giovanni Salerno (Abstract)

The angle of a side-chain decides regio- and enantioselectivity in Alcohol Dehydrogenase A, Thilak Reddy Enugala, Emil Hamnevik, Dirk Maurer, Heidi Hillier, Doreen Dobritzsch, and Mikael Widersten (Abstract)

Directed evolution of artificial metalloenzyme – in vivo catalysis, Shreyans Chordia and Gerard Roelfes (Abstract)

Exploring the promiscuity of LmrR as a scaffold for artificial metalloenzymes, Cora Gutiérrez, Ivana Drienovska, Lara Villarino, and Gerard Roelfes (Abstract)

A fluorescent hydrogel-based flow cytometry screening platform for hydrolytic enzymes, Volkan Besirlioglu, Christian Pitzler, Ljubica Vojcic, Ronny Martinez, Ulrich Schwaneberg, Georgette Wirtz, Stephanie Hiltl, and Alexander Böker (Abstract)

Discovery and development of novel glucanotransferases for healthier foods, Tim Börner, Joana Gangoit, Lubbert Dijkhuizen, S. van Leeuwen, Lisa Lamothe, Christina Vafiadi, and Stéphane Duboux (Abstract)

Engineering 2’O-mRNA methyltransferases for industrial biocatalysis, Pierre-Yves Colin, Paul A. Dalby, Mary Stahley, and Jared Davis (Abstract)

Engineering better quorum quenching enzymes, Thomas James Wiggins, Monica Gerth, and Suzanne Warring (Abstract)

Effect of additional domains on the elongation mechanism and fructosyl linkage specificity of the multidomain levansucrase LevS, Flor de María García-Paz, Sandra Morales-Arrieta, and Clarita Olvera-Carranza (Abstract)

Papaya lipases heterologous expression: Towards structure and function relationship, Georgina Sandoval (Abstract)

A novel atomistic motional correlation method combined with thermodynamics to delineate the intricate mechanism of substrate specific catalysis: Enzyme engineering perspective, Naveen Kulkarni, Devashish Das, and R.Pravin Kumar (Abstract)

Molecular cloning and Biochemical properties of GH-16 β-agarase from Gilvimarinus agarolyticus JEA5, Youngdeuk Lee, Eunyoung Jo, Chulhong Oh, and Do-Hyung Kang (Abstract)

Biochemical properties of a novel neoagarotriose-producing β-agarase from Gilvimarinus agarolyticus JEA5, Eunyoung Jo, Youngdeuk Lee, Su-Jin Lee, Chulhong Oh, and Sachithra Amarin Hettiarachchi (Abstract)

Recombinant protein production in Escherichia coli by combining of signal peptide originated from Bacillus subtilis, Chulhong Oh, Youngdeuk Lee, Su-Jin Lee, Eunyoung Jo, Sachithra Amarin Hettiarachchi, and Do-Hyung Kang (Abstract)

A newly identified glutaminase-free ʟ-asparaginase (ʟ-ASPG86) from the marine bacterium Mesoflavibacter zeaxanthinifaciens, Su-Jin Lee, Youngdeuk Lee, Eunyoung Jo, Eunyoung Jo, and Sachithra Amarin Hettiarachchi (Abstract)

Synergistic effect of acetyl xylan esterase on xylanase reaction originated from Ochrovirga pacifica, Sachithra Amarin Hettiarachchi, Su-Jin Lee, Youngdeuk Lee, Do-Hyung Kang, Chulhong Oh, and Eunyoung Jo (Abstract)

Development of screening method for the selection of mutants to improve the substrate specificity of Pyrococcus furiosus thermostable amylase, Nan-Young Lee, Jong-Tae Park, Eun-Ji Park, and Cheul-Soon Yim (Abstract)

Improving bread quality using Deinococcus geothermalis glycogen branching enzyme, Eun-Ji Park, Cheul-Soon Yim, Nan-Young Lee, and Jong-Tae Park (Abstract)

Improving activity of an N-glycosyltransferase using a medium throughput HPLC assay, Timothy G. Keys (Abstract)

FireProt: Web server for automated design of thermostable proteins, Radka Chaloupkova, Milos Musil, Jan Stourac, Jaroslav Bendl, Jan Brezovsky, Zbynek Prokop, Radka Chaloupkova, Tomas Martinek, and David Bednar (Abstract)

CAVERDOCK: A new tool for analysis of ligand binding and unbinding based on molecular docking, Radka C haloupkova, Jiri Filipovic, Ondrej Vavra, Jan Plhak, David Bednar, Sergio Marques, Jan Brezovsky, Radka Chaloupkova, and Ludek Matyska (Abstract)

HotSpot Wizard 3.0: Automated design of site-specific mutations and smart libraries, Radka Chaloupkova, Lenka Sumbalova, Jan Stourac, Radka Chaloupkova, Tomas Martinek, and David Bednar (Abstract)

Marine DNA polymerases as tools for next generation molecular diagnostics solutions, Yvonne Piotrowski, Olav Lanes, Magnus Seppola, and Atle Noralf Larsen (Abstract)

Multifunctional enzyme engineering by computational design for lignocellulosic valorization, Claire Dumon, Claire Dumon, David Bryant, Michael O’ Donohue, Narcis Fernandez-Fuentes, and Eleni Ioannou (Abstract)

Construction of a secondary metabolite deficient penicillium chrysogenum strain as a generic production host for secondary metabolites, Fabiola Polli, Annarita Viaggian, Oleksander Salo, Peter Lankhorst, Rob van der Hoeven, Roel. A. L. Bovenberg, Dick Janssen, and Arnold J. M. Driessen (Abstract)

Glycodiversification: Glycosynthases towards variation of flavonoid glycosides, Marc Richard Hayes, Lothar Elling, Kazuhito Fujiyama, Takao Ohashi, and Jörg Pietruszka (Abstract)

Protein engineering of Candida rugosa lipase, Satoru Ishihara, Tetsuya Takahashi, Satoshi Koikeda, Kazunori Yoshida, and Kazuhiko Ishikawa (Abstract)

Artificial ligninolytic secretome by S. cerevisiae: Building a white-rot yeast, David Gonzalez-Perez and Miguel Alcalde (Abstract)

Exploring donor substrate promiscuity of a Thermostable Transketolase by directed evolution, Thangavelu Saravanan and Wolf-Dieter Fessner (Abstract)

Tailored biosynthesis of plant-derived ginsenoside Rh2 in yeast via repurposing a key promiscuous microbial enzyme, Yan Feng, Yu Zhuang, Guangyu Yangg, and Xueli Zhang (Abstract)