ECI Digital Archives

Title

Cofactor switch: Development of A Nad+-dependent cascade for the production of ursodeoxycholic acid (UDCA)

Authors

Fabio Tonin, TU Delft, NetherlandsFollow
Linda G. Otten, Department of Biotechnology, Delft University of Technology
Isabel W.C.E. Arends, Department of Biotechnology, Delft University of Technology
Ulf Hanefeld, Department of Biotechnology, Delft University of Technology

Conference Dates

September 15-19, 2019

Abstract

The employment of alcohol dehydrogenases in cascade reactions is often limited by the different cofactor specificity of the enzymes involved: the employment of additional cofactor regeneration systems and the excess amount of sacrificial substrates frequently increase the environmental impact and the costs of biocatalytic processes. Additionally, a NADP⁺-dependent process is generally less desirable, inasmuch this cofactor is more expensive, unstable and less naturally available than NAD⁺, leading to an increase of the process costs.

Nowadays, protein engineering offers the possibility to switch the cofactor dependency of enzymes introducing few targeted mutation¹.

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Recommended Citation

Fabio Tonin, Linda G. Otten, Isabel W.C.E. Arends, and Ulf Hanefeld, "Cofactor switch: Development of A Nad+-dependent cascade for the production of ursodeoxycholic acid (UDCA)" in "Enzyme Engineering XXV", Huimin Zhao, University of Illinois at Urbana-Champaign, USA John Wong, Pfizer, USA Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/enzyme_xxv/78