Conference Dates
June 12 – 17, 2022
Abstract
Enveloped virus-like particles (eVLPs) are a complementary strategy for the manufacturing of vaccines against enveloped viruses. The baculovirus expression vector system (BEVS) in insect cells is an attractive and widely used platform for the expression of eVLPs. Inherent to this system is the co-expression of baculoviruses and other extracellular vesicles, which we could nicely demonstrate by high resolution electron microscopy on one of our expression supernatants (Figure 1). Such heterogenous sample mixtures pose a major bottleneck for effective downstream processing of insect cell-expressed enveloped virus-like particles. eVLPs, baculoviruses and extracellular vesicles are similar in size and share a lot of common surface properties which make chromatographic separation difficult. In addition, immune responses against baculoviruses have been reported in humans, therefore efficient removal is mandatory.
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Recommended Citation
Alois Jungbauer, Katrin Reiter, Patricia Aguilar, Miriam Klausberger, Florian Strobl, Wolfgang Ernst, Gerald Striedner, and Reingard Grabherr, "Production of influenza virus-like particles by insect cells and removal of baculovirus from virus-like particles and other extracellular vesicles" in "Vaccine Technology VIII", Tarit Mukhopadhyay, Merck Research Laboratories, USA; Charles Lutsch, Sanofi Pasteur, France; Linda Hwee-Lin Lua, University of Queensland, Australia; Francesc Godia, Universitat Autònoma de Barcelona, Spain Eds, ECI Symposium Series, (2022). https://dc.engconfintl.org/vaccine_viii/129