April 23-28, 2023
Cancun, Mexico

Chairs:   Laura A. Palomares, Instituto de Biotecnología, UNAM, Mexico
  Chetan Goudar, Amgen, USA
  Tongtong Wang, Genentech, USA

The articles for these proceedings are not peer-reviewed.



Conference program - Cell Culture Engineering XVIII, Laura A. Palomares, Chetan Goudar, and Tongtong Wang (Article)

“Cell Culture Engineering” and what this means for the future of medicine, Alison Moore (Abstract)

Human gene therapy – Principles, history, state of the art, challenges and approaches, Guangping Gao (Abstract)

Insights into bispecific antibody development, characterization, and manufacture, Latonia Harris and Pedro Alfonso, Ph.D. (Abstract)

Continuous improvement in bispecifics manufacturing: Advantages and disadvantages of 2-cell vs. 1-cell culture process, Jessica Wuu (Abstract)

Regeneron bispecific molecule structure begets platform production process compatibility, Shawn Lawrence, Hanne Bak, Mark Czeterko, Brandon Veres, Michelle LaFond, and Gang Chen (Abstract)


Challenges and successes in developing and manufacturing multiple formats of bispecific antibodies, Weichang Zhou (Abstract and Presentation)

Transforming molecule selection and process development through attribute focus and the deployment of high-performance computing tools, Neeraj Agrawal (Abstract)

Cellular demands of secreted protein products – systems and synthetic biology improve quality and titer, Johan Rockberg, Magdalena Malm, Rasool Saghaleyni, Niklas Thalén, Chih-Chung Kuo, Veronique Chotteau, and Nathan E. Lewis (Abstract)

Building one block at a time toward complex biologics using cell-free protein synthesis process, Marcella Yu (Abstract)

Cell line technologies for speed-to-clinic and commercial production of biologics, Gang Chen (Abstract)

Newly-established Chinese hamster-derived cell line for protein production, Takeshi Omasa, Noriko Yamano-Adachi, and Takayuki Horiuchi (Abstract and Presentation)

Engineering of Chinese hamster ovary cell lipid metabolism results in an expanded ER and enhanced recombinant biotherapeutic protein production, James Budge, Tanya J Knight, Joanne Roobol, Ian R Brown, Jane Povey, Gurdeep Singh, Colin M Jaques, Sarah Turner, Robert J Young, Andrew J Racher, Andrew Dean, and C Mark Smales (Abstract)

Chromosomal instability drives convergent and divergent evolution toward advantageous inherited traits in mammalian CHO bioproduction lineages, Meiping Chang (Abstract)

What's in a Phenotype?, Nicole Borth, Martina Baumann, Marcus Weinguny, Peter Eisenhut, Nicolas Marx, Heena Dhiman, Gerald Klanert, Neza Novak, Giulia Borsi, Winston Timp, Daniel Ivansson, Andreas Jonsson, Ann Lovgren, and Isac Lee (Abstract)

Systems and synthetic biology approaches towards optimization of N- glycan sialylation, Jack Scarcelli (Abstract)

What does a cell need for efficient protein secretion: Deciphering, modeling, and augmenting the CHO machinery, Nathan Lewis, Helen O. Masson, Mojtaba Samoudi, Mina Wu, Chih-Chung Kuo, Caressa Robinson, Magdelena Malm, and Johan Rockberg (Abstract)

GalMAX: Model-inspired glycoengineering for biopharmaceutical quality assurance, Ioscani Jiménez del Val, Itzcoatl Gomez Aquino, Alfonso Blanco, Sara Carrillo, and Jonathan Bones (Abstract)

Synthetic cell lines for recombinant AAV production, Wei-Shou Hu, Zion Lee, Wen Cai, Yu-Chieh Lin, Ibrahim Irfanullah, and Min Lu (Abstract)

Feedback-responsive cell factories for biomanufacturing, Laura Segatori (Abstract)

The microRNA landscape of the extracellular vesicles generated by Chinese hamster ovary cells under normal and stressed conditions, Eleftherios Papoutsakis, Jessica Belliveau, and Will Thompson (Abstract)

Metabolic engineering of high-productivity CHO host lines for biomanufacturing, Jamey Young, Sarah A. Sacco, Kevin J. Ruiz-Márquez, Lauren Kraft, Kevin D. Smith, and Michael J. Betenbaugh (Abstract)

Biologics 4.0: Emergence of the CHO Biofoundry, Lars Nielsen and Lise Marie Grav (Abstract)

Transcriptomics guided mechanistic metabolic model for perfusion culture process, Veronique Chotteau, Meeri Mäkinen, évin Colin, Hubert Schwarz, Magnus Lundqvist, Johan Rockberg, Håkan Hjalmarsson, and Veronique Chotteau (Abstract)

Effective cell culture operations by implementing accurate, non-invasive determination of the critical process parameter pH in Roche`s Drug Substance Network, Christian Klinger (Abstract)

Real-time data-driven and multi-scale model-guided system for bioproccess digital twin platform, Dong-Yup Lee, Seo-Young Park, Cheol-Hwan Park, Sun-Jong Kim, Jinsung Song, and Dong-Hyuk Choi (Abstract)

Upstream control strategy development for afucosylated species in mAb biomanufacturing, Jianlin Xu, Khandaker Siddiquee, Adam Rish, Zhuangrong Huang, Yashika Singh, Johanna Santos, Jianfa Ou, Emily Rittershaus, Xuankuo Xu, Grace Yao, Melissa Holstein, Kitty Agarwal, Gregory Keil, Linda Hoshan, May Ou, Lakshmi Kandari, Ji Zheng, Dan Huang, Gabi Tremml, Michael Borys, Sanchayita Ghose, and Anurag Khetan (Abstract)

Bioreactor scale induced alteration in cell metabolic state can impact amino acid misincorporations in recombinant proteins produced in CHO cells, Shanta Boddapati, Kyle Boone, Sofie O’Brien, Howard Clarke, Aaron Chen, John-Vallerie Douglass, and Jason Gilmore (Abstract)

Machine learning and advanced data analytics automating the exploitation of Raman spectroscopy: From micro-scale to large-scale operation, Stephen Goldrick, Matthew Banner, Michael Thomas, Jonathon Welsh, and Suzanne S. Farid (Abstract)

ADVANCED CONTROL OF GLYCOSYLATION AND TITER IN FED-BATCH MONOCLONAL ANTIBODY PRODUCTION, Anne Robinson, Yu Luo, Devesh Radhakrishnan, Evan A. Wells, Liqing Song, Varghese Kurian, and Babatunde A. Ogunnaike (Abstract)

Martin Sinacore Award Lectures - Accelerated process development and commercialization - Bringing life- saving drugs to market, Pooja Jambunathan (Abstract)

Mechanistic insights into N-glycosylation microheterogeneity of recombinant proteins produced in cell culture, Madhuresh Sumit, John J.. Scarcelli, John J. . Scarcelli, and Bhanu. Chandra Mulukutla (Abstract)

Rapid, scalable, cost-effective process for generation of stably integrated chimeric antigen receptor (CAR) engineered T-cells by “Gene Writing”: An all RNA approach, without need for use of viral vectors or nucleases, Madhusudan V. Peshwa (Abstract)

AI-enabled biomanufacturing, Stephen Balakirsky (Abstract)

Adaptive T cell processing through integrated process analytical technologies, Ivie Aifuwa (Abstract)

Differential effects on natural killer cell production by membrane‐bound cytokine stimulations, Zhimei Du, Meiping Chang, Luke Nelson, Xiaoyan Tang, and Gregg Nyberg (Abstract)

A soft sensor of cell concentration in a perfusion bioreactor via a digital twin, Joseph Egan, Núria Marí-Buyé, Elia Vallejo, Miquel Costa, David Horna, and Stephen Goldrick (Abstract)

Advancing manufacture of hiPSC-derived hepatocytes with improved functionality: A nature-inspired protocol, Margarida Serra, Joana I. Almeida, Inês E. Crespo, Nikolaus Virgolini, Daniel Pais, Inês A. Isidro, Paula M. Alves, Pedro Vicente, Tomásl Calmeiro, Elvira Fortunato, CENIMA T|i3N, Henrique V. Almeida, Maren Calleja, Juan R. Rodriguez-Madoz, Felipe Prosper, and Pedro M. Baptista (Abstract)

Process analytical utility of Raman spectroscopy for cell therapy manufacturing, James Piret, H. Georg Schulze, Shreyas Rangan, Martha Z. Vardaki, Katherine MacDonald, Diepiriye G . Iworima, Megan K. Levings, Timothy J. Kieffer, Michael W. Blades, and Robin F. B. Turner (Abstract)

Engineered T-cell therapy: State of the science, Susan Abu-Absi (Abstract)

Multispecificity -- The future of molecular medicines, Ray Deshaies (Abstract)

Advancing the productivity, robustness, and scalability of AAV production process by transient transfection in suspension cell culture, Jenny Shupe (Article)

A hot new bioprocess strategy to improve small EV production, Kerstin Otte, Nadine Hornung, Oliver Hertel, Raimund Hoffrogge, and Kerstin Otte (Abstract)

Development of a Subclonal host cell line for AAV production, Saurabh Sen (Abstract)

Development of a second-generation lentiviral vector to reduce COGM while meeting both vector and CAR T cell CQAs, Julia Proctor, Ashley Baltes, Dorothy Maxwell, and John Moscariello (Abstract)

Rapid cell culture process optimization and scale-up for production of two replicating viral vector COVID-19 vaccine candidates, James Wagner, Daniel Spatafore, Adam Kristopeit, and Justin Ma (Abstract)

Overcoming barriers in viral vector manufacturing: Small molecule targeting of antiviral defences, Jean-Simon Diallo, Keara Sutherland, Naveen Haribabu, Ella Korets-Smith, Jondavid de Jong, and Andrea Vervoort (Abstract)

Maintaining productivity over extended durations for perfusion processes, Paul Gramlich, Supal Vyas, Kristi Daris, and Pheng Yam (Abstract)

Scaleable microscale perfusion systems for yeast and mammalian cells to accelerate process development of bioproducts, María del Carme Pons Royo, Peter Satzer, and Diethard Mattanovich (Abstract)

On digital bioprocessing for manufacturing intelligence: Application of process analytical technology (PAT) and process data analytics (PDA) for upstream process development and intensification, Ricardo Suárez-Heredia, Amos Lu, Victoria Berger, Christian Airiau, Kevin Brower, Marina Hincapie, and Ethan Penner (Abstract)

Development of a highly concentrated perfusion medium supplement to decrease media demand leveraging a newly designed 250 mL single use perfusion bioreactor, Mona Bausch, Luis Ayala, Channing McLaurin, Lisa Wolowczyk, Jana Mahadevan, Jochen B. Sieck, and Long Pham (Abstract)

Ultra-intensified intermittent-perfusion fed-batch (UIIPFB) process quadrupled productivity of a bispecific antibody, Jun Tian, Le Yu, Weichang Zhou, Wesley Wang, and Jinliang Zhang (Abstract)

CCE Award Lecture - Cell Culture and Social Engineering, Manuel Carrondo (Abstract)

Case study: Optimization stage of a bispecific DART® molecule identified dissolved carbon dioxide (dCO2) is a critical upstream process parameter for growth, productivity and scalability, Nathalie Gerassimov, Nicholas Barpoulis, Hugh Graham, and Senthil Sivagurunathan (Abstract)

Adeno-associated virus production in suspension cell culture using Eppendorf bioprocess control systems and BioBLU® single-use bioreactors, David Solbach (Abstract)

The impact of expression vector position on transgene transcription allows for rational expression vector design in a targeted integration system, Kathryn Beal (Abstract)

Co-culturing cell lines for efficient manufacture of multispecifics, Dawn Eriksen-Stapleton, Tim Iskra, Lia Ingaharro, Josh Ochocki, Michael King, Dhruv Srivastava, and Guogang Dong (Abstract)

Analysis of production bottlenecks in BiTE® molecules producing CHO cells, Tobias Jerabek, Madina Burkhart, Jochen Pendzialek, Anika Menthe, Ruediger Neef, and Kerstin Otte (Abstract)

From nanoliter to large-scale bioreactors: How integrated technologies bring antibody treatments to patients faster, Véronique Lecault, Lucas Kraft, Fabian A. Garces, Harveer Dhupar, Fatima Ahmed, Valentine de Puyraimond, Franziska von Bank, Della Keohan, Stefanie Žentelis, Shauna M. Crowley, Erica Lovett, Kathryn Westendorf, Rodrigo Goya, Yuri Hwang, Roza Bidshahri, Iliana Lanz, Lisa Anderson, David W. Collins, Rahul Rana, Kevin Jepson, Robin van der Lee, Matthew Wiggin, Maia A. Smith, Carl Hansen, Ester Falconer, Bryan C. Barnhart, Amanda Moreira, Emilie Lameignere, Jens Ruschmann, Kush Dalal, Ping Xiang, Davide Pellacani, and Rod Docking (Abstract)

Fast track fed-batch culture development for COVID-19 vaccine clinical study, Joon-Chul Lee, Jeanho Park, Yong-Jin Kim, Na-young Hong, Junki Ahn, Rock-ki Kim, Kyung-ho Kim, and Young-Ok Baek (Abstract)

Polyclonal production of antibodies using CHO cell line mixtures, Anna Christina Adams, Lise Marie Grav, Andreas Hougaard Laustsen, and Lars Keld Nielsen (Abstract)

Designing a highly productive next generation CHO fed batch platform, Kyle McElearney, Jian Wu, Juan Parra, Hannah Fitch, Brian McConnell, Jessica Kotini, Niloy Chakma, Orry Nihangame, Kristi Daris, Huong Le, and Natalia Gomez (Abstract)

Rescue protein production with effector gene determination in CHO cells, Ece Cagdas, Johan B latt Rojek, and Lise Grav (Abstract)

Design, expression, and characterization of an asymmetric bispecific antibody fused to a scFv shuttle for brain uptake, Juan C arlos Rivera and Laura A. Palomares (Abstract)

Designing a CHO protein production platform using multi-omics technology, Chengjian Tu, Anna-Barbara Hachmann, Megan Pajak-Lee, Fatima Zara, Alexander Fox, Mary Reynolds, and James W. Brooks (Abstract)

Streamlining T cell engager development with a diverse panel of fully human CD3-binding antibodies, bispecific engineering technology, and an integrated discovery engine, Swapnil Bhargava, Bryan C Barnhart, Lindsay DeVorkin, Yuri Hwang, Cristina Faralla, Wei Wei, Emma Lathouwers, Raffi Tonikian, Karine Herve, Tim M Jacobs, Kate Caldwell, Juntao (Matt) Mai, Sherie Duncan, Kush Dalal, and Aaron Yamniuk (Abstract)

Mitigating development risk of complex modalities during early-stage cell line development, Ren Liu and Fnu Aapjeet (Abstract)

Fucosylation inhibitor development for producing afucosylated antibodies, Lakshmi Kandari, Ping t Xu, May Ou, Michael Smith, Michael Schmidt, Rodney Parsons, and Anurag t Khetan (Abstract)

A production-adapted, multi-auxotrophic, CHO cell line for reducing monoclonal and bispecific antibody cell line development timelines, James Ravellette, David Razafsky, Jason Gustin, Trissa Borgschulte, and Mary Otto (Abstract)

High throughput analysis for cell line development, Saloni Khurana, Christina Tsai, and Marvin Peterson (Abstract)

Process for generation of high-producing CHO cell lines for biomanufacturing of biologics using our CHOrcTA platform, Simon Joubert, François Vaillancourt, Félix Malenfant, Julie Guimond, Sylvie Perret, Julie Guimond, Phuong Lan Pham, Robert Voyer, Yves Durocher, and Julien Leroy (Abstract)

Identifying early predictors of instability in CHO cell lines using the Berkley Lights Beacon® Optofluidic System, James Donaldson, Caroline Wardrope, and Susan Rosser (Abstract)

Engineering Chinese Hamster Ovary cells for enhanced protein secretion, Mauro Torres and Alan J. Dickson (Abstract)

Power of platform to accelerate therapies beyond a pandemic, Ronan Kelly and Christopher Carl Frye (Abstract)

Engineering CHO cell lines for the production of biosimilars of murine cell derived reference products, Shivani Gupta, Bhavana Shah, Chetan Goudar, and James M. Pire (Abstract)

Novel directed evolution platform for creating high-productivity cells for therapeutic protein production, virus production, and other processes, Larry Forman, Kathy Ngo, and Lawrence Chasin (Abstract)

A novel system for glycosylation engineering by natural and artificial micrornas, Kerstin Otte, Florian Klingler, Friedemann Hesse, Patrick Schlossbauer, Lukas Naumann, and Christian Neusüß (Abstract)

Fine tuning of plasmid design to generate tailored CHO cell lines for production of bi- and multispecifics, Anett Ritter and Dattananda Chelur (Abstract)

Rebuilding CHO again and again: Development of a species agnostic modular cell line development platform for cultivated meat, Kim Le, Michaela Walker, Ara Hwang, Kevin Kayser, and Mark Juhas (Abstract)

Modulation of sialic acid content for enzyme fusions using combinatorial approach of cell line engineering and process development, Yashas Rajendra, Melina Lenser, Anh Nguyen Dang, Julie Ngo, and Allain Petersen (Abstract)

High yielding large-scale transient expression of Biotherapeutics in CHO: Beyond preclinical development, Anurag Khetan, Kitty Agarwal, Juan Camilo Gonzalez Rivera, Todd Ryder, Lakshmi Kandari, and Monica George (Abstract)

Omics-based characterization of random transgene integration sites from CHO production cell lines, Sofie O'Brien, Neha Patel, Jodi Grantham, Howard Clarke, and Jake Leinas (Abstract)

High ER content and upregulated UPR pathway render a unique CHO host with high antibody productivity and product quality, Ren Liu (Abstract)

Engineered transposon for improved cell line development, Kristi Daris, Neeraj J. A Agrawal, Natalia Gomez, Jennitte Stevens, Noelia Blanco-Talavan, Huong Le, Trupti Guhan, and Lynette Buck (Abstract)

Characterization of extrachromosomal circular DNA (eccDNA) structure, function, and dynamics in a CHO K-1 clone, Dylan Chitwood, Qinghua Wang, Stephanie R. Klaubert, Kiana Green, Cathy Wu, Sarah W. Harcum, and Christopher A. Saski (Abstract)

Identification of transgene integration sites, their structure and epigenetic status with Cas9-targeted nanopore sequencing in CHO cells, Nicolas Marx, Klaus Leitner, Krishna Motheramgari, and Nicole Borth (Abstract)

Genome-wide CRISPR-Cas9 screen identifies hyperosmotic stress responsive genes in Chinese hamster ovary cells, Su Hyun Kim, Seunghyeon Shin, Minhye Baek, Kai Xiong, Karen Julie la Cour Karottki, Hooman Hefzi, Lise Marie Grav, Lasse Ebdrup Pedersen, Helene Faustrup Kildegaard, Nathan E. Lewis, Jae Seong Lee, and Gyun Min Lee (Abstract)

NextGen Sequencing (NGS) platform and applications in cell line development, Ying Shen (Abstract)

Characterization of neutralizing human anti-tetanus monoclonal antibodies produced by stable cell lines, Ana Maria Moro, Daniela Takat, Tania Manieri, Wagner Quintilio, and João Batalha-Carvalho (Abstract)

Finding the ideal: Single-cell cloning and seamless media transitions, Michelle Sabourin, Zhou Jiang, and Paula Ravnikar (Abstract)

Automation of a cell culture process with lager vessels, Ingo Focken, Tibor Anderlei, Dave . Laidlaw, and Ralph Kuppelwieser (Abstract)

Enhancing recombinant protein and viral vector production in mammalian cells by targeting the YTHDF readers of N6–methyladenosine in Mrna, Niall Barron and Nga Lao (Abstract)

CRISPR activation screening of dormant genes to improve secretory capacity in CHO cells, Johan Blatt Rojek, Giulia Scapin, Che Lin Kim, and Lasse Ebdrup Pedersen (Abstract)

Dynamic regulation of mitochondrial metabolism as a strategy to maximize mAb production in industrial CHO cell cultures, Kevin Ruiz-Marquez, Sarah A . Sacco, Lauren Kraft, Michael J. Betenbaugh, and Jamey D. Young (Abstract)

Transposase-mediated transgene integration for rapid generation of high-producing stable cell lines, Lauren Kraft, Elizabeth Sorichillo, and Thomas Kelly (Abstract)

Applying synthetic biology and computational biology to advance biologics expression platforms, HaeWon Chung, Adam Carcella, Brianna Jayanthi, Casey O'Connor, Kevin D. Smith, Dinghai Zheng, Alec A.K. Nielsen, and Akash Gopaluni (Abstract)

Development of a high throughput CHO cell glycosylation enzyme Mrna expression profiler, Shivani Gupta, Chetan Goudar, and James M. Piret (Abstract)

Use of stable CHO pools and CHO TGE to accelerate SARS-CoV-2 vaccine development, Yves Durocher; Matthew Stuible; Simon Joubert; Sergio Alpuche; Simon Lord-Dufour; Zalma Vanesa Sanchez Martinez,; Louis Bisson; Janelle Sauvageau; Jason Baardsnes; Mauro Acchione; Majida El Bakkouri; Joseph Schrag; and Denis L’Abbé (Abstract)

Novel cell engineering platform for increasing specific productivity of CHO cells for therapeutic antibody production in fed-batch cultures, Kathy Ngo, Lawrence Chasin, and Larry Forman (Abstract)

Cell lines development through targeted integration involving a CRISPR-Mad7-based system in CHO-DG44, Dubhe Beatriz Bulte Ocaña, Lise M. Grav, Alexandra Baer, and Peter Ravn (Abstract)