April 23-28, 2023
Cancun, Mexico

Chairs:   Laura A. Palomares, Instituto de Biotecnología, UNAM, Mexico
  Chetan Goudar, Amgen, USA
  Tongtong Wang, Genentech, USA

The articles for these proceedings are not peer-reviewed.


Transcriptional and metabolic response of CHO cells to different carbon dioxide concentrations, Jorge Campano, Alberto Porras, Octavio T. Ramírez, and Laura Palomares (Abstract)

Tracking amino acid metabolism in CHO cell cultures using stable isotope labeling assisted metabolomics, Maciek Antoniewicz, Harnish Mukesh Naik, Venkata Gayatri Dhara, Jacqueline E. Gonzalez, Eleanor Oates, Swetha Kumar, and Michael Betenbaugh (Abstract)

Developing methodologies to optimise production of recombinant antibodies at specific phases of Chinese Hamster Ovary (CHO) cell culture, Ellie Hawke, Mauro Torres, Alan J Dickson, Alison Young, and Robyn Hoare (Abstract)

Model-based scale-up of CHO cell culture process, Emmanuel Anane, Nina Moenster, and Christoffer Bro (Abstract)

Understanding the effect of oxidative and ER stress on CHO cell culture and product quality through multivariate analysis, Leran Mao, Anne S. Robinson, and James W. Schneider (Abstract)

Lifecycle management of a commercial platform monoclonal antibody process: The promise of ICHQ12, Cillian McCabe, Deirdre Buckley, and Noreen Lynch (Abstract)

Combined effect of ammonia stress and cell line age on CHO cell derived VRC01 monoclonal antibody glycosylation, Nicholas Sandoval, Benjamin Synoground, Kathryn Elliott, Claire McGraw, Jada Roth, and Sarah W. Harcum (Abstract)

DNA sequence to a million doses of COVID-19 therapeutic antibody in 9 months – Making the seemingly impossible, possible, Matthew D. Osborne, Yao-Ming Huang, Jennifer Purdie, and Robert O’Keeffe (Abstract)

Characterization of cellular viability drop during media and platform development, Vince Price, Lauren M. Kraft, and Kevin Clark (Abstract)

Expression of stress proteins during fed batch and perfusion cultures, Claudia Berdugo, Oscar Lara-Velasco, Kevin Humbard, Eric Plencner, and Jeffrey Chalmers (Abstract)

Real-time bioprocess and automated feed control with in-line Raman sensor, Ching-Jen Yang, Chao-Yi Teng, and Ming-Hung Hsu (Abstract)

Measuring Golgi's redox potential in CHO cells and its response to hypoxic conditions, Stfanny Wendy Meza Soto, Frania Zúñiga Bañuelos, Laura A. Palomares, and Octavio T. Ramírez (Abstract)

Mixing and mass transfer in shake flasks and their relationship with antibodies productivity of CHO cells, Mauricio Trujillo-Roldan, Saumel Pérez-Rodríguez, and Norma A. Valdez-Cruz (Abstract)

Generation and characterization of a process-specific HCP reagent using stably transfected Null Cell Pools, Sven Loebrich, Muhammad Arshad Chaudhry, and Wilhad Hans Reuter (Abstract)

High density culture of human induced pluripotent stem cells in stirred tank bioreactors for regenerative therapies, Jorge Escobar, Felix Manstein, Nils Kriedemann, Wiebke Triebert, Robert Zweigerdt, and Kevin Ullmann (Abstract)

From product microheterogeneity to homogeneity using integrated modeling methodology and new cell culture component, Bassem Ben Yahia, Valentine Chevallier, and Laetitia Malphette (Abstract)

Transient expression of CHIKV VLP in large stirred tank bioreactors, Peifeng Chen and Daniel Gowetski (Abstract)

Automation of an N-1 perfusion process using BioCapacitance, Abbey Weith, Dan Trout, Stef Berges, and Kevin Clark (Abstract)

Multifunctional surface modifications to capture and expand flowing endothelial colony- forming cells, Corinne A. Hoesli, Mohamed A Elkhodiry, Julia Manalil, Omar S Bashth, Jean-François Tanguay, and Gaétan Laroche (Abstract)

Design and in vitro validation of smart microcarriers for next generation cell culture, Hugo Level, Anita Kriz, Marc-Antoine Campeau, and Prof. Corinne Hoesli (Abstract)

Proof-of-concept of a novel scalable magnetic bead-based cell separation technology, Nils Brechmann, Kristofer Eriksson, Torbjörn Pettersson, and Véronique Chotteau (Abstract)

Immunocompetent human 3D brain cell cultures to advance ATMP development, Catarina Brito, Catarina M Gomes, Daniel Simão, Gabriela Silva, Maria João C Sebastião, Patrícia Gomes-Alves, and Paula M Alves (Abstract)

Novel vertical-wheel bioreactors for the scalable, robust and efficient production of shear- sensitive cell therapy products – human pluripotent stem cells (aggregate culture) and mesenchymal stromal cells (microcarrier culture), Matthew Croughan, Sunghoon Jung, Omokhowa Agbojo, Hannah Worden, Maximilian Lee, Brian Lee, and Breanna S. Borys (Abstract)

Encapsulation of mesenchymal stem cells and MCF-7 cancer cells in aqueous two-phase systems droplets for 3D culture applications, Marco Rito-Palomares, Karolina Chairez-Cantu, and Mirna González-González (Abstract)

Integration of pancreatic aggregates into BioSilk network to treat diabetes, Kelly Blust, Siqin Wu, Carolina Åstrand, and My Hedhammar (Abstract)

Development of a dynamic SH-SY5Y 3D culture model for biological evaluation of Alzheimer-induced pathology, Macarena Fernandez, Alejandra Arancibia, Constanza Núñez, Mauro Torres, Mauricio Vergara, and Claudia Altamirano (Abstract)

Novel cell engineering platform for improving production of AAV for gene therapy applications, Kathy Ngo, Paul Escarpe, Lawrence Chasin, and Larry Forman (Abstract)

Environmental footprint of adherent cell culture viral vector drug substance manufacturing process, Morgan Soukup (Abstract)

Advancing large-scale production of MSC-derived extracellular vesicles through cellular pre-conditioning and enhanced process control, Margarida Serra, Marta Costa, Inês Carrondo, and Enrique Oltra (Abstract)

A scalable and flexible solution for robust T cell expansion, Jeffrey Mulkin, Mitchell Maloy, Chris Brau, Evan Zynda, Kyle Zastrow, and Tom Mellody (Abstract)

Simple and convenient method for adipose-derived mesenchymal stem cells serum-free medium development, Joon-Chul Lee, Hyejeong Heo, Yong-Jin Kim, and Joon-Chul Lee (Abstract)

Novel impeller design for stem cell bioprocessing and its application in hMSC stirred-tank bioreactor cultures, Tom Adam Wyrobnik, Alan Lam, Steve Oh, Andrea Ducci, and Martina Micheletti (Abstract)

Towards the design of a feeder-free system for natural killer cell expansion, Janani Narayan, Pedram Motallebnejad, Wei-Shou Hu, Samira M. Azarin, Frank Cichocki, and Jennifer One (Abstract)

Ex vivo expansion and differentiation of erythroblasts: From culture dishes to stirred tank reactors for the production of red blood cells, George Barringer,; J. Gallego Murillo; T. van Arragon; L. van der Wielen; E. van den Akker; and A. Wahl (Abstract)

Cell therapy process development with a 2 mL perfusion bioreactor, Ryan Trocki, Kevin S. Lee, Wei-Xiang Sin, Harry Lee, and Michael Birnbaum (Abstract)

Utilisation of capacitance measurement (dielectric spectroscopy) to monitor, control and improve viral vector and virus-based vaccine production, Aditya Bhat (Abstract)

The role of extracellular vesicles in the modulation of the cell density effect, Jesús Lavado García, Pol Pérez-Rubio, Laia Bosch-Molist, Elianet Lorenzo-Romero, Laura Cervera, and Francesc Gòdia (Abstract)

Continuous harvesting of retroviral murine leukemia viral vector particles in a high cell density perfusion cultivation, Marc Dominique Hein, Daniel Kazenmaier, Yasemin van Heuvel, Tanya Dogra, Yvonne Genzel, Sascha Y. Kupke, Jörn Stitz, and Udo Reichl (Abstract)

Influenza A virus OP7 defective interfering particles: Cell culture-based production and antiviral efficacy in vivo, Lars Pelz, T Dogra, JD Boehme, M Baelkner, J Kuechler, MD Hein, P M arichal-Gallardo, Y Genzel, D Bruder, SY Kupke, and U Reichl (Abstract)

Scaleable transient transfection of intensified rAAV production processes, Kathryn Olson and Jeffrey Pavlicek (Abstract)

A hot new bioprocess strategy to improve small EV production, Christoph Keysberg, Nadine Hornung, Oliver Hertel, Raimund Hoffrogge, and Kerstin Otte (Abstract)

Towards large-scale production of human-induced pluripotent stem cell-derived extracellular vesicles in stirred-tank bioreactors, Ana M eliciano, Ana Filipa Louro, Pedro Vicente, Paula Marques Alves, Margarida Serra, and Cláudia Diniz (Abstract)

Simplifying manufacturing and control of dual vector AAV therapies, Peter Slade (Abstract)

Expression of anti-apoptotic genes to enhance rAAV production, David Catalán-Tatjer, Saravana Kumar Ganesan, Jesús Lavado-García, Lise Marie Grav, Ivan Martínez-Monge, and Lars Keld Nielsen (Abstract)


On the AAVenue to success: Advances in technologies for AAV production, Sandra Klausing, Niklas Kraemer, Julian Droste, Stefan Northoff, Tim Steffens, Franziska Sundermann, Vera Ortseifen, Alyssa Vetter, and Kathrin Teschner (Abstract and Presentation)

Exploring alternative oncogene-free mammalian hosts for adeno-associated virus production, Seongkyu Yoon, Nelson Ndahiro, Junneng Wen, Hung Tran, Seongkyu Yoon, Ashli Polanco, and Michael Betenbaugh (Abstract)

Design space determination and process optimization of product quality attributes in transient rAAV manufacturing, Seongkyu Yoon, Qiang Fu, Yong Suk Lee, Erica A. Green, Yongdan Wang, So Young Park, Kelvin H. Lee, Michael Betenbaugh, and David McNally (Abstract)

Process improvements for AAV production by transient transfection of HEK293 cells, Stephanie Doong, Patrick Bohse, Thomas Gagliardi, Rahul Chelikani, and Anita Shrestha (Abstract)

Hybrid liposome-extracellular vesciles for targeted delivery of CRISPR/Cas9, Jessica Belliveau, Samik Das, and E. Terry Papoutsakis (Abstract)

High cell density perfusion process for rAAV9 production based on transient transfection of HEK293 cells, Brian Ladd, Ye Zhang, Emil S undäng Peters, and Véronique Chotteau (Abstract)

Accelerating the Vero vaccine production platform through the generation of high-density cell banks and direct thaw into microcarrier bioreactor cultures, Samantha Marrone, Christopher Ton, Brianna Frederick, Nickolas Swisher, David Adler, and Jillian Shingler (Abstract)

The effect of cell density on the plasmid utilization for the production of adeno-associated virus via the triple-transfection method, Brian Ladd, Veronique Chotteau, and Torbjörn Gräslund (Abstract)

Suspension like scalability of rAAV9 production in adherent cells, Brian Ladd, Veronique Chotteau, Torbjörn Gräslund, and Andreas Castan (Abstract)

Intensified virus production in suspension HEK293SF cells using high inoculum fed-batch or low-perfusion rate cultures with continuous harvest, Cristina Silva, Amine A Kamen, and Olivier Henry (Abstract)

Scalable iPSC-based platform to produce tissue-specific Extracellular Vesicles, Rachel Moen, Hayden Pagendarm, Jamey Young, Ethan Lippmann, Alissa Weaver, John Wilson, and Youn Jae Jung (Abstract)

Scale up of Sf9/BEVS-based AAV production process for GMP-manufacturing of cardiovascular gene therapies, Tae Won Chung (Abstract)

Medium- and high-cell-density production of Adeno-Associated virus serotype 6 and the mitigation of cell density effect via medium supplementation, Pablo Diego Moco, Cristina A. T. Silva, and Amine Kamen (Abstract)

Conditioned medium derived from murine BM-MSCs cultured as spheroids exhibit in vitro immunomodulatory capacity, Claudia Altamirano, Paloma . Fuentes, María . José Torres, and Mauricio . Vergara (Abstract)

Process development and scaleup comparisons for transient production of AAV, Michael Gillmeister and Don . Startt (Abstract)

Developing rAAV production platform with enhanced productivity, scalability and biosafety, LiYing Yang, Longxiang Sheng, Ran Li, Bo Liu, and Huapeng Li (Abstract)

Improved production of viru like particles of Cowpea Chlorotic Mottle virus using the baculovirus expression system, Anayeli Martínez, Ruth Pastor, Octavio T. Ramírez, and Laura Palomares (Abstract)

HSC70/HSP40 overexpression in viral vector-producing Sf9 cells impacts AAV productivity and product quality, Juan Aponte-Ubillus, Jim Liu, Daniel Barajas, Joseph Peltier, and Javier Femenia (Abstract)

Large scale production and purification of a lentiviral vector reference material, Otto-Wilhelm Merten, Aziza Manceur, Anja Rodenbrock, Sushma Puttaswamaiah, Edward Kwee, Jakob Reiser, and Keith L. Carson (Abstract)

Improving recombinant Adeno-Associated Virus production through plasmid design and host cell line optimization, Jenny Shupe, Soundarya Kandala, Terrence Dobrowsky, and Yizhou Zhou (Abstract)

Integrated semi-continuous manufacturing of lentiviral vectors, Michelle Yen Tran and Amine A. Kamen (Abstract)

On demand, in-situ media and buffer preparation in continuous integrated biomanufacturing to reduce water consumption and CO2 footprint, Alois Jungbauer, Daniel Komuczki, Tommaso De Santis, and Peter Satzer (Abstract)

Leucine and Isoleucine derivatives as bioavailable alternatives for canonical amino acids in cell culture media to enable next generation bioprocesses, Tim Hofmann, Corinna Schmidt, Dmitry Zabezhinsky, and Aline Zimmer (Abstract)

Implementation of N-1 process intensification in bioprocess development and production, Jochen Schaub, Andreas Ankenbauer, Sabine Arnold, Matthias Brunner, Tobias Habicher, Nicolas Maguire, Michael Löffler, Dominique Monteil, Lisa Stepper, Andreas Unsöld, Julia Walther, Fabian Stiefel, and Thomas Wucherpfennig (Abstract)

Enabling commercial-scale perfusion manufacturing using single-use bioreactors and tangential flow filtration, Nicholas Guros, Michael Mollet, Ken Lee, and Alex Gadberry (Abstract)

A scalable single-use perfusion system: Are single-use bioreactors suitable & scalable?, Quang Long Pham, Channing McLaurin, and Jana Mahadevan (Abstract)

Scale-up of cell culture processes using Digital Twins, Mehran Rafigh, Pranjul Mishra, Kevin Schindler, Pulkit . Chhabra, Joachim Schmid, Shilpa . Nargund, and Klaus Mauch (Abstract)

Toolbox for efficient development of high cell density perfusion process, integrated with continuous DSP, Nils Arnold Brechmann, Hubert Schwarz, Meeri E.L. Mäkinen, Veronique Chotteau, Joaquín Gomis Fons, Bernt Nilsson, Madelène Isaksson, Julia Scheffel, Sophia Hober, and Andreas Castan (Abstract)

Improving AAV manufacturing by integrated and continuous processing, João P. Mendes, Antonio Roldao, Cristina Peixoto, and Ricardo J.S. Silva (Abstract)

Insect cell-based production processes intensified via high cell density perfusion and continuous culture, Antonio Roldao, Bárbara Fernandes, Ricardo Correia, Inês Isidro, and Paula M. Alves (Abstract)

Raman spectroscopy for on-line monitoring of amino acid concentrations and antibody N- glycosylation in high cell density perfusion process, Veronique Chotteau, Hubert Schwarz, Meeri Mäkinen, and Andreas Castan (Abstract)

Development and application of an advanced PAT strategy to an integrated continuous drug substance manufacturing process, Chao-Hsiang Wu, Chetan Goudar, Neil Soice, Nitin Rathore, Gang Xue, Becky Chan, Jette Wypych, and Rohini Deshpande (Abstract)

Development and scale-up of perfusion cell culture process for higer productivity, Hirokatsu Makitsubo, Nobumasa Takao, Wataru Tanaka, Kiyoshi Hirakawa, Yasuharu Shinoda, Atsushi Kuwahara, Haruka Sano, Yuta Hasunuma, Ryota Nakajima, and Akihiko Ban (Abstract)

Study of the magnetic properties of glioblastoma cancer stem-like cells and non-stem tumor cells using magnetophoresis for label-less separation, James Kim, Monica Venere, and Jeffrey J. Chalmers (Abstract)

Fully automated on demand cell culture media preparation for perfusion, Lisa Wolowczyk, Christiant Schultheiss, Jochen Sieck, and Mona Bausch (Abstract)

Development of Scale-down system for perfusion precesses, Bjarne Rask Poulsen, Martin Heitmann, Nicolas Marx, and Claes Nymand Nilsson (Abstract)

Marching toward implementation of an ultra-high density dynamic perfusion process, Dominique Monteil, Nikta Farsaei, Marcella Yu, Marlene Castor-Melchor, Scott Godfrey, and Daisie Ogawa (Abstract)

Oxygen transfer rate model for cell-free and predictive D.O. control in intensified bioreactor processes, Samantha Whitney, Jonathan Cain, John A Muckstadt, Allyson Caron, Amy Wood, Brandon Medeiros, and Haley Matthews (Abstract)

Process design of a fully integrated continuous biopharmaceutical process using economic and ecological impact assessment, Chaoying Ding, Marianthi Ierapetritou, Hiren Ardeshna, and Christopher Gillespie (Abstract)

Economic and ecological analysis of plastic-degrading enzyme production, Tommaso De Santis and Gerald Striedner (Abstract)

Use of Ambr15 as a high throughput model to speed up perfusion bioprocess development, Xiaoxia Jin, Matthew Stauffer, Zhenya Hu, Lia Tescione, and Canghai Lu (Abstract)

Scalability of CO2 stripping efficiency from bench (3 L) to pilot scale (200 L) for supporting intensified bioprocesses, Samantha Whitney, Marisa Maher, and Amy Wood (Abstract)

Strategies for small-scale perfusion cultures in ambr®250 HT bioreactor system, Srikanth R apala, Abiageal Barton, Sarah W. Harcum, and Kathryn S. Elliot (Abstract)

Operational enhancements to improve applicability for challenging cell lines in the Sanofi Intensified Perfusion Platform (IPeP), William N. Napoli, Brooke Coughlan, Shawn Barrett, and Jared Franklin (Abstract)

Advancements on process development and transfer for Integrated Continuous Biomanufacturing, David Garcia, Nuno Carinhas, Aleksandr Pogodaev, Jörg Altekrueger, Mengyao Li, Tanja Hernandez, and Thorsten Lorenz (Abstract)