October 19-24, 2025
Helsinger, Denmark
| Chairs: | John Woodley, DTU |
|---|---|
| Ditte Hededam-Welner, DTU |
25 conference program - Enzyme Engineering XXVIII, John Woodley, Ditte Hededam Welner, Jens Erik Nielsen, and Technical Liaison (Article)
Insights into enzyme catalysis and engineering for sustainable synthetic biology, Pimchai Chaiyen (Abstract)
Computational design of high-efficiency enzymes, Sarel Fleishman (Abstract)
Digital enzyme evolution: Creation of a fully digitalized enzyme engineering platform, demonstrated through the multiparameter engineering of imine reductases (IREDs), Andrew Currin (Abstract and Presentation)
Enzymes of the future are built bottom – up: SAM-independent methyltransferases, Matthew Thompson (Abstract and Presentation)
Computational design of efficient enzymes through coevolution and correlation-mediated allosteric networks, Silvia Osuna, Cristina Duran, and Guillem Casadevall (Abstract)
Capturing protein dynamics and its determinants using explainable artificial intelligence, Faraneh Haddadi, Joan Planas-Iglesias, Jiri Damborsky, and Stanislav Mazurenko (Abstract)
Benchmarking novel AI platforms against in silico designs: Enzymes for low – sugar foods, David Schönauer, Eduardo Oliveira, and Lynn Schwardmann (Abstract)
Enzyme engineering for sustainable processes and products, Volker Sieber (Abstract)
From lab to industry: How enzyme stability can help drive the green transition through innovative biosolutions, Lars Giger (Abstract)
Biocatalytic methylation in drug discovery, Diana Amariei, Pascal Schneider, Mikhail Kabeshov, Felix Faber, Lucy Harwood, Agnes Ingvarsson, Martin Hayes, and Christof Jäger (Abstract)
Enzymatic synthesis in deep eutectic solvents: Catalysis across hydrophilic and hydrophobic realms, Selin Kara, Ningning Zhang, Viktoria Lahmann, Jan Philipp Bittner, Pablo Domínguez de María, Sven Jakobtorweihen, and Irina Smirnova (Abstract)
Engineering formate dehydrogenase for applications in biocatalysis, Carlos G. Acevedo-Rocha, Samuel A. Bradley, Nadja Eisenbruch, Aina Pages I Lara, Anna Maria Walerowicz, Rasmus Kamp Nielsen, Mehmet Mervan Çakar, Niklas Gesmar Madsen, and Artur Luchian (Abstract)
Engineering promiscuous aryl acylamidases for pesticide bioremediation, Thierry Marti, Diana Schweizer, Milo Schärer, Yaochun Yu, Serina L. Robinson, and Silke Probst (Abstract and Presentation)
Process readiness of biocatalysts: Redox reactions and amide couplings, Andy S. Bommarius (Abstract)
Enzymes for sustainable biomanufacturing: Expanding the molecular repertoire and getting there faster, Ee Lui Ang, Wan Lin Yeo, Dillon W. P. Tay, Jhoann M.T. Miyajima, Shreyas Supekar, Tong Mei The, Jin . Xu, Yee Ling Tan, Jie Yang See, Hao Fan, Sebastian Maurer-Stroh, and Yee Hwee Lim (Abstract)
Engineering enzymes for RNA oligonucleotide manufacturing, Anders Knight (Abstract)
Enzyme engineering for the application of biocatalysts under non- physiological reaction conditions, Dörte Rother (Abstract)
Engineered enzymes at pH 11.5 to achieve a 200-fold volume reduction in API synthesis, Pravin Kumar, Mukesh Kumar, Pradeepa A, Shilpa J, Rakshanaa Rajagopal, Ashwin Sundar, Pravin Kumar R, Roopa Lalitha, and Gladstone Sigamani (Abstract)
Engineering of halohydrin dehalogenase HHEG for application in microflow, Anett Schallmey, Marcel Staar, and Lina Ahlborn (Abstract)
Designing and engineering protein scaffolds for topologically complex metallocofactors, Jared Lewis (Abstract)
Integrated strain, enzyme and bioprocess design to bring progress to life, René de Jong (Abstract)
Increasing mevalonate kinase activity by mutating a variable active site residue identified in natural variant alignments, Sylvia Sarnick, Joel L. Kaar, Daniel K. Schwartz, and Yannick J. Bomble (Abstract and Presentation)
In vitro biocatalytic synthesis of nicotine alkaloids, Gideon Grogan, Benjamin Schwabe, Zoe Ingold, Martin Fascione, and Benjamin Lichman (Abstract)
AI-guided enzyme cascade engineering for sustainable production of glucaric acid, Haibin Chen; Hu . Hu; Zhongjie Hong; Baoqin Cai; Ameng Zhou; and Tianyun Cai, (Abstract)
Distal mutations in a designed retro-aldolase alter loop dynamics to shift and accelerate the rate-limiting step, Cindy Klaus, Serena E. Hunt, Aqza E. John, Niayesh Zarifi, Alec Martinez, Ferran Feixas, Marc Garcia-Borràs, Michael C. Thompson, and Roberto A. Chica (Abstract and Presentation)
Improving protein functionality by combining experiment and computation, Kresten Lindorff-Larsen (Abstract)
Biocatalytic synthesis of non-natural peptides, Juan E Valásquez (Abstract)
Clean solutions from dirty genes: Discovery and engineering of plastic –degrading enzymes from soil eDNA libraries, David Ackerley, Hannah R. Lee-Harwood, Anna G.C. Moore, Alistair S. Brown, and Amy G. Alexander (Abstract)
Accelerating discovery of substrate promiscuity in biocatalyzed oxidations, Joelle Pelletier (Abstract)
Protection from oxidative damage by hole hopping drives evolution in lytic polysaccharide monooxygenases (LPMOs), Tom Emrich-Mills, Iván Ayuso-Fernández, Ole Golten, Zarah Forsberg, Kelsi Hall, László Nagy, Morten Sørlie, Åsmund Røhr Kjendseth, and Vincent G.H (Abstract)
Analysing enzyme surface mobility kinetics in fabric and home care, Charlotte Stenkjaer Fletcher, Nazar Momin, and William G. T. Willats (Abstract)
Growth-based selection: From non-canonical amino acids to defluorinases – and back again, Clemens Mayer (Abstract and Presentation)
Protein design 2.0, Birte Höcker (Abstract)
Stable and highly active knotted DHFR through computational design and directed evolution, Yajie Liu, Jing Fang, and Wen-Bin Zhang (Abstract)
Building enzymes with new function, Anthony Green (Abstract)
Rational enzyme design in amino acid – based biocatalysis, Francesca Paradisi (Abstract)
Enzymatic synthesis of complex human milk oligosaccharides, Birgitte Zeuner (Abstract)
Directed enzyme discovery: A new paradigm for rational biocatalyst identification, Maria Fatima Lucas (Abstract)
The 3DM AI platform can accurately combine mutations into a small number of higher clones with massive performance increase, Henk-Jan Joosten (Abstract)
Thiolase functionality as a key driver for sustainable biochemical production, Hyeoncheol Son (Abstract)
Development of protein redesign approaches informed by molecular evolutionary theory, Shogo Nakano, Hiroki Ozawa, Taichi Chisuga, and Shoryu Fujita (Abstract)
Results of the first Protein Engineering tournament: A detailed analysis of the generative round and comparison to Industrial Protein Engineering methods, David Estell (Abstract)
Accessible biocatalyst development by Rapid In vitro Semi-rational Engineering (RISE) of enzymes, András Telek, Viktor Gaják, Gergő Dargó, Jan Mican, David Bednar, Beáta G. Vértessy, and Gábor T asnádi (Abstract and Presentation)
Fast and scalable directed evolution of a methyltransferase family by in silico stability prediction, Lucas Bocquin and Stephan C. Hammer (Abstract and Presentation)
Engineering formate dehydrogenase for applications in biocatalysis, Carlos Acevedo-Rocha, Samuel A. Bradley, Niklas Gesmar Madsen, Artur Luchian, Mehmet Mervan Çakar, Rasmus Kamp Nielsen, Anna Maria Walerowicz, Aina Pages I Lara, and Nadja Eisenbruch (Abstract)
Global transcription factor engineering for designing robust microbial cell factories, Yosephine Gumulya, Yuwei Xiang, Esteban Marcellin, and Mark S Turner (Abstract)
VMF: Disruptive Vibro® technology for Enzymes biomanufacturing processes, Jarno Robin (Abstract)
Machine learning guided directed evolution of an unspecific peroxygenase, Li Wan and Martin J. Weissenborn (Abstract)
Novel aldolase-like enzymes from mechanism coupled phage display, Mikael Widersten, Annika Söderholm, Thomas Norberg, Joel Leufstadius, and Annette Roos (Abstract)
An enzyme switch based on luciferase activated by antigen-triggered release of its fragment fused to an antibody, Takanobu Yasuda, Masahiko Taguchi, Shun Kagamihara, Tetsuya Kitaguchi, and Bo Zhu (Abstract)
Machine learning for scalable molecular dynamics and evolution-aware protein design, Pavel Kohout, Guillem Casadevall, Jiri Damborsky, Sílvia Osuna, and Stanislav Mazurenko (Abstract and Presentation)
What neutrons can do for you: The single crystal neutron diffractometer BIODIFF at the Heinz Maier-Leibnitz Zentrum (MLZ), Tobias Erich Schrader and ndreas Ostermann (Abstract)
Directed enzyme discovery: A new paradigm for rational biocatalyst identification, Maria Fatima Lucas (Abstract)
Novel enzyme systems in gut microbial dietary component metabolisms useful for health promotion, Jun Ogawa, Michiki Takeuch, Shigenobu Kishino, Ryotaro Hara, and Akinori Ando (Abstract)
How hydrophobic interfaces challenge enzyme stability, Maria Isabel Rodriguez Torres, Elif Erdem, Marina Y. Linova, and John M. Woodley (Abstract)
Utilizing split inteins for post-translational creation of multi-catalytic enzymes, Tove Widén, Linus Storm, Johan Larsbrink, and Daniel Krska (Abstract)
Rapid and iterative deep mutational scanning via selection campaigns enables AI-assisted enzyme engineering, Gol Mohammad Dorrazehi, Matthew Penner, Joe Perkins, Monika Papworth, and Florian Hollfelder (Abstract)
Innovative approaches to GAPDH modification for efficient NADPH-driven chemical biosynthesis, Hyeoncheol Son (Abstract)
Oxidative biocatalysis at higher temperatures: Effect of temperature on mass transfer at 2L and 200L scales, Emilie Overgaard Willer, Panagiotis Chasapis, Juliet J. Victoria, and John M. Woodley (Abstract)
A simple method to measure kinetics of oxygen-dependent enzymes, Ariadna Pié Porta, Elif Erdem, and John M. Woodley (Abstract)
Ancestral sequence reconstruction as a tool for structural analysis of modular polyketide synthases, Taichi Chisuga, Akimasa Miyanaga, and Shogo Nakano (Abstract)
Sweet, salty & hot: First insights into archaeal UDP-dependent glycosyltransferases from extreme habitats, Onur Kırtel and Ditte Hededam Welner (Abstract)
Structure determination and enzyme engineering for simple amino acid analysis method, Hiroki Yamaguchi, Kazutoshi Takahashi, Moemi Tatsumi, and Masayuki Sugiki (Abstract)
Synthesis of modified nucleotides via engineered biocatalytic enzymes, Ericka Bermudez (Abstract)
Kgo-catalyzed oxyfunctionalization of industry-relevant substrate classes, David Schoenauer, Eduardo Oliveira, Bogdan . Barz, and Hannah Brass (Abstract)
Metagenomic discovery of portable resistance enzymes for next-generation cancer therapies, Abigail Sharrock, Alex Linton-de Boer, Moana Tercel, Rob Weinkove, and David Ackerley (Abstract)
Direct fermentative production of sarcosine by engineered corynebacterium glutamicum with sam- dependent glycyne-n-methyltransferase, Riku Usami, Jun Takakura, Yohei Tatsukami, and Jun Ogawa (Abstract)
Salt-dependent Taqman Qpcr: A temperature independent approach to diagnostic amplification, Shalus Singh, Winston Koh, Farid J Ghadessy, and Seow Yiqi (Abstract)
Enzyme engineering for improving thermostability of endolysin, Sasina Premjaichon, Penchit Chitnumsub, Ubolsree Leartsakulpanich, and Pimchai Chaiyen (Abstract and Presentation)
Enzymatic DNA Synthesis: the new gold standard for ssDNA synthesis, gene assembly and enzyme engineering, Manon Molina, Benoit Derrien, Mikhael Soskine, Elise Champion, and Xavier Godron (Abstract)
Engineering of an ochratoxin A degrading enzyme for efficient performance in animals, Sebastian Fruhauf, Christoph Gonaus, Ioannis Zachos, and Gerd Schatzmayr (Abstract)
A machine learning approach for faster discovery of oxidizing enzymes for difficult chemical reactions, Lilly Marie Eger, Felix Moorhoff, Ludger A. Wessjohann, Mehdi D. Davari*, and Martin Dippe (Abstract and Presentation)
Metal promiscuity in present-day and ancestral terpene cyclases, Pauline Granit, David Sjönell, Karen Schriever, David Hueting, Erik Sundén, and Assoc. Prof. Per-Olof Syrén (Abstract)
Enzymatic characterization and kinetic analysis of monomeric variants derived from homodimeric amylosucrase, Yun-Sang So, Dong-Ho Seo, and Sang-Ho Yoo (Abstract)
Machine learning-guided engineering of galactose oxidase for enhanced activity on non-galactose carbohydrate substrates, Julia Gordijo, Jose Barraza Chavez, Xuebin Feng, Prof. Emma Master, and Benjamin Sanchez (Abstract and Presentation)
Indigoidine synthetases; Driving the synthesis of novel C-nucleosides and pigments, Alistair Brown, Amy Alexander, and David Ackerley (Abstract)
Reprogramming poly(A) polymerase for single-nucleotide extension of RNA oligonucleotides, Joonyoung Lee, Seong-Gyu Lee, Jin Young Kang, and Ki Jun Jeong (Abstract)
Engineering oxygen tolerance in anaerobic CO dehydrogenase via Fe-S cluster stabilization and multilayer O₂ tunnel sealing, Jingu Kang, Suk Min Kim, So Yeon Kong, and Sung Heuck Kang (Abstract)
How much Flavin is too much? Exploring cofactor driven control of Oxidase activity and lifetime, Elif Erdem, Maria Isabel Rodriguez-Torres, Volker Sieber, and John M. Woodley (Abstract)
Optimizing whole-cell catalysis of 2,5-furandicarboxylic acid (FDCA), Anna Z. Schuppe, Ximena Lopez-Lorenzo, and Per-Olof Syrén (Abstract)
Application of a high-throughput selection to enrich for plastic-degrading enzymes from metagenome libraries, Hannah Lee-Harwood, Dr Alistair Brown, Prof David Ackerley, and Anna Moore (Abstract)
LMCO2, a novel laccase for PE degradation, Chiara Tantillo, Nina Gruschwitz, and Per-Olof Syrén (Abstract)
Simulation of pH gradients in a 200 L reactor during biocatalytic ester hydrolysis, Caroline Hamelmann, Juliet Victoria, Ulrich Krühne, John M. Kavanagh, David F. Fletcher, and John M. Woodley (Abstract)
MD-guided salt bridge analysis and pKa engineering to enhance acidic stability of ChCODH, Doa Kim, YoungSeob Lee, and Yong Hwan Kim (Abstract)
Enzymatic denim dyeing with biocatalytic indican, Mandy Hobusch, Ruben Marcel De Boer, Inês Gil, Tsai-Ting Shih, and Ditte Hededam Welner (Abstract)
Engineering of NADH oxidases for efficient NAD+ regeneration in enzymatic cascades, Franka Matena, John Woodley, and Volker Sieber (Abstract)
Utilizing split inteins for post-translational creation of multi-catalytic enzymes, Tove Widén, Linus Storm, Johan Larsbrink, and Daniel Krska (Abstract)
Design, synthesis and directed evolution of single-domain dihydrofolate reductase catenanes, Jing Fang, Yajie Liu, and Wenbin Zhang (Abstract)
Enzyme-based multiplex biosensors for biosynthetic intermediates, Yoshiki Takemura, Yuki KIMURA, and Daisuke UMENO (Abstract and Presentation)
Redesigning specificity of betaine synthase using substrate-induced stabilization, Haruki Uryu, Yuki KIMURA, Daisuke UMENO, and Taro Watanabe (Abstract and Presentation)
Novel antioxidant enzymatic functions are part of the Clic protein family’s repertoire of moonlighting activities, Stella Valenzuela, Khondker Rufaka Hossain, Wendy El Khoury, Daniel Turkewitz, and Amani Alghalayini (Abstract)
ProteusAI: An open-source and user-friendly platform for machine learning-guided protein design and engineering, Carlos Acevedo-Rocha, Jonathan Funk, Laura Machado, Samuel A. Bradley, Marta Napiorkowska, Patrick V. Phaneuf, Henry Webel, Niklas G. Madsen, Liubov Pashkova, Rodrigo Gallegos-Dextre, and Timothy P. Jenkins (Abstract)
Systematic dissection of the unique epimeric selectivity of DdGal1Pase in Tagatose biosynthesis via metadynamics bootstrapping, Pravin Kumar, Sukesh Narayan Kashyap, Dhinesh Kumar Santhamoorthy, Roopa Lalitha, Gladstone Sigamani, Aaron M. Love, Christopher G. Toomey, Liwei Li, Parayil Kumaran Ajikumar, Nikhil U. Nair, Christine Nicole S. Santos, and Pravin Kumar (Abstract)
Cross-Structure Energy Mapping Identifies A279R Mutation Enhancing Fluorinase Functionality and Oligomerization, Pravin Kumar, Anuj J Shetty, Likith Muthuraj, Michaela Slanska, Daniel C. Volke, Isabel Pardo, Roopa Lalitha, Gladstone Sigamani, Jiri Damborsky, Pablo Ivan Nikel, Zbynek Prokop, and Pravin Kumar R (Abstract)
Identification and characterization of niclosamide-active glycosyltransferases, Martina Escorial García, Brianna Marie Lax, Onur Kırtel, and Ditte Hededam Welner (Abstract)
Enzymatic detoxification of deoxynivalenol (DON) for low-impact agriculture, William Olsen, Jacob Gjøderum Koch, Valeria Della Gala, Marko Vasiljević, Jog Raj, Hunor Farkaš, Onur Kırtel, and Ditte Welner (Abstract)
Directed evolution of a transferase for SAM analog (re)generation from simple bromoalkanes, Friederike Reese, Marius Schnutenhaus, and Stephan C. Hammer (Abstract)