April 27 - May 2, 2025
Tucson, AZ
| Chairs: | A Khetan, BMS |
|---|---|
| Marcella Yu, Gilead | |
| Mike Betenbaugh, Johns Hopkins University |
Increasing mAb productivities in CHO cells by manipulating adenosine update- novel insights into cell growth and productivity regulation, Sri Madabhushi, Tomali Chakravarty, Tomas Kasza, Balrina Gupta, Tariq Bassam Atieh, and Malik Padellan (Abstract)
Evolution of platforms with emerging technologies: Application of an intensified process with a focus on product quality, Yang Yang, Jackie Gonzalez, Joe Yakamavich, Stefan Romeder-Finger, Terrence Dobrowsky, and Zoe Horton (Abstract)
Innovative and accelerated approach towards cell culture media raw material control strategy to increase manufacturing process robustness of biologics, Delia Lyons, Amr Ali, Andrew Bawn, Clayton Wandishin, Sean McDermott, Kevin Ortiz-Rivera, Chuanzi Oung, David Robinson, and Nicholas Abu-Absi (Abstract)
Next generation transient protein expression, Divya Goel, Amita Goel, and Divya Goel (Abstract)
Machine learning driven (Bio)process exploration, Matteo Planchestainer, Corentin Briat, Colin Jaques, and Thomas K. Villiger (Abstract)
Novel PAT solutions enable precise, real-time feed control for improved mAb product quality profiles, Stephanie Klaubert, Awab Nehela, Katherine Raiche, Milla Neffling, Scott E. Miller, Graziella Piras, and Erin Redman (Abstract)
Platform development for a dynamic perfusion process and performance across scales, Aravindan Rajendran, Jenn Nguyen, Wenge Wang, Matt Gagnon, and Alaina Anand (Abstract)
Development of a continuous manufacturing process to control product quality with representative scale-up performance, Tiffany Tang, Joseph Fantuzzo, Balveen Wasan, Xiaolin Zhang, Hassan Raza, Joshua Polevoy, Alexis Quinteros, Michael Brian Deck, Jason Houser, Zachary Kasica, Chelsea Phuangthong, Tariq Atieh, and Nuno D.S. Pinto (Abstract)
Enhancing AAV production using tunable molecular dials in HEK-293 producer cell lines, Kathy Ngo, Anson Shai, Larry Forman, and Srinivas Kalanghad (Abstract)
From single cell cloning to perfusion: combined AI-driven and hybrid clone selection model for cost-effective and time-efficient cell culture and bioprocessing, Franziska Stefurak, Dr.-Ing Ali Safari, Timo Schmidberger, Lukas Klein, Dr. Kristin Thiele, and Dr. Dirk Müller (Abstract)
Uncovering mechanisms of high rAAV production using genome-wide CRISPR screens, José Escandell, Filipa Moura, Paula Alves, Patricia Alves, and Mariana Antunes (Abstract)
Mapping Cellular Processes that Determine Delivery of Plasmid DNA into the Nucleus: Application in Mammalian Expression Host Cells to Enhance Recombinant Protein Production, James Budge (Abstract)
Technical development of the Ambr® 250 mini bioreactor to optimise performance for late-stage process development, Francesco Mancuso, Ellen Lam, Shaker Sabah, Gregory Casee, Kerbrat Maina, Andrew Tait, and Barney Zoro (Abstract and Presentation)
Maduramycin and kifunensine for modulating high mannose glycoforms in antibody-based protein therapeutics, Nandita V ishwanathan, Thomas Vuillemin, Patrick Romann, Martin Jordan, Jean-Marc Bielser, and Thomas K. Villiger (Abstract)
Single cell transcriptomics identifies limitations in transfection-based adeno-associated virus production process, Sofia Tunmats, Brian Ladd, Torbjörn Gräslund, and Veronique Chotteau (Abstract)
Stress and success: Understanding NK cell production through single-cell analysis, Brian Ladd, Markella Zacharouli, Stefanie Renken, Per-Henrik Holmqvist, and Veronique Chotteau (Abstract)
Enhancing rAAV yield through mixer-based optimization of transient transfection for scalable gene therapy production, William Larsson, William Larsson, Torbjörn Gräslund, and Veronique Chotteau (Abstract)
Molecular mechanisms of lactate metabolic shift in CHO cells: Role of ATF4 pathway, Mauro Torres, Leon Pybus, Ellie Hawke, Alison Young, and Alan J. Dickson (Abstract)
Monitoring recombinant adeno-associated virus production in mammalian cell cultures via single-cell biophysical profiling and machine learning, Francesco Destro, Weida Wu, John Joseph, Paul W. Barone, Stacy L. Springs, Anthony J. Sinskey, Richard D. Braatz, Scott R. Manalis, and Amogh Morje (Abstract)
Improving mAb production in a fed-batch process: A paradigm shift in CHO cell culture, Mitch Raith, Michael Giampapa, Pritam Sinha Roy, and Alexandra Squeri (Abstract)
Combining directed evolution with multi-omics analysis for host cell engineering, Sarika Mehra and Priya Mishra (Abstract)
Cell and product specific synthetic genetic components and assemblies for biopharmaceutical production and gene therapy, David James (Abstract)
Integrating Raman spectroscopy, multiarray fluorescent and data-driven soft sensors for enhanced real-time monitoring within bioprocess digital twins, Dong-Yup Lee, Seo-Young Park, Cheol-Hwan Park, Sun-Jong Kim, Jinsung Song, Dong-Hyuk Choi, Youngwook Cho, and Sooyeon Cho (Abstract)
Leveraging orthogonal transposase/transposon pairs as an alternative genetic engineering tool in CHO cells, Mário Pereira, Oren Beske, Ferenc Boldog, and Jeremy Minshull (Abstract)
Enabling automation, data management, and in silico tools for cell culture development, Kyle McHugh, Terrence Dobrowsky, Yang Yang, Nate Frazee, and Stefan Romeder-Finger (Abstract)
Enhancing production and purification of adenovirus-like particles (ADDomer) for snakebite therapy, Antonio Roldao, Raquel Arinto-Garcia, Birhanu Hurisa, Cláudia Paiva, Emerson Dilla, Justin Sargunas, Ricardo Correia, and Cristina Peixoto (Abstract)
Mass transfer modeling of dissolved carbon dioxide levels across scales and sites, Sanjeev Ahuja and Kevin Stone (Abstract)
Novel cell engineering platform yielding highly productive CHO cells for therapeutic antibody manufacturing, Larry Forman, Kathy Ngo, James Dal Santo, Hsin-Yi Li, and Anson Shai (Abstract)
AI-driven lead clone selection for enhanced cell line development, Stephen Goldrick (Abstract)
Playing defense and offense: Scale down models and process solutions to mitigate protein reduction 1, Ryan Sanford, Valerie Pferdeort, and Sarwat Khattak (Abstract)
Mitigation of LMW impurities via feed optimization in a high-titer fed batch process, Daniela Espinosa-Hoyos, Valerie Besset, Yingnan Si, Stephanie Riley, Dylan Rapoport, Xhuliana Picari, William N. Napoli, Amos Lu, Rachel Kapelner, Priyanka Joshi, Agathe Drape, Brooke Coughlan, and Rachel Van Corbach (Abstract)
Enhancing deep-well plate cultures for predictive bioprocess development, Cheong Xin Ooi, Nikesh Patel, Christina Maag, Matthew Arango, Markus Kaiser, Matthew Demers, Mina Rafieishishavan, Aditya Tulsyan, Mohammad Rashedi, Bhushan Gopaluni, and James M. Piret (Abstract)
Transcriptomics-guided design of a feeder-free culture system for NK cell expansion, Janani Narayan, Pedram Motallebnejad, Frank Cichocki, Wei-Shou Hu, and Samira M. Azarin (Abstract)
Process development approaches for engineered B cell medicines, a new class of cellular medicines, Alan Gilbert, Mark Lalli, Rashmi Kshirsagar, and Andre Stanlie (Abstract)
Feeding TCA Cycle Intermediates for pH control improves CHO cell culture performance and increases TCA cycle flux, Sandra Bennum, Kevin J. Ruiz-Marquez, Irina Trenary, Rachel Moen, Xiaolin Zhang, Henry Lin, Malik Padellan, Xiaowen Wang, and Jamey D . Young (Abstract)
Impact of PAT ultra-low continuous glucose control on mAb Product Quality and CHO culture performance, Awab Nehela, Stephanie R. Klaubert, Erin Redman, Katherine Raiche, Scott E. Miller, Milla Neffling, and Graziella Piras (Abstract)
Cell culture optimization through metabolic modeling and metabolomics in cellular agriculture, Pomaikaimaikalani Yamaguchi, Kirsten Trinidad, Kyongbum Lee, and David Kaplan (Abstract)
Celia: A model-based platform for media and feeding strategy design for improved process metrics, Ziomara Gerdtzen, Iván Paredes, Aaron Canales, Bastián Herrera, Francisca Pizarro, Isidora Prat, Temístocles Molina, Ana Quiroga Campano, and J. Cristian Salgado (Abstract)
Pilot scale integrated dynamic bioprocessing - scaling perfusion technology, Alex Gadberry, Ashna Dhingra, Nicholas Guros, Elise Woodall, Anita Kundu, and Ana Dilillo (Abstract)
High-throughput screening for high performance cell culture media development, Shan Gao, Matthew Tenorio, Chandana Sharma, David T. Ho, Omid Taghavian, Yosuke Kurokawa, Xiaofei Wang, and Hamid Soleymani (Abstract and Presentation)
Scalable and automated bioprocessing strategies for intensified viral vector production in Hek293 cell culture, Omid Taghavian, Jose R. Sanchez, Shan Gao, Hamid Soleymani, Katsuko Sato, Chandana Sharma, Francisco Franco, Masafumi Nishino, and Nozomu Tonoike (Abstract and Presentation)
Identification and epigenetic induction of co-fluctuating heritable gene expression states for activating stress tolerance in CHO, Spencer Grissom, Kayla Wolf, Mark Blenner, and Abhyudai Singh (Abstract)
Early screening of clones based mitochondrial capacity and cellular redox for improved productivity and lactate accumulation, Malini Ahuja, Michael Badlowski, Anurag Khetan, Dan Huang, and Megan Schmidt (Abstract)
Process Intensification without N-1 Perfusion: A case study on process and media development for a CHO cell line, Andrew Hu, Aditya Gopalan, and Martin Gawlitze (Abstract and Presentation)
Using Ambr250 as a scale down model to accelerate late-stage process development and process characterization / process validation for a Chinese hamster ovary cell culture process, Anh Nguyen Dang, William Bobbitt, Andrew Hu, Martin Gawlitzek, and Barry Wolf (Abstract)
Exploring non-oncogenic hTERT cell lines as alternative AAV producers, Hung Tran, Qiang Fu, Ashli Polanco, Soyoung Park, and Seongkyu Yoon (Abstract)
Subcloning of HEK293T cells increases lentiviral vector production, Kim Curtis, Zhou Jiang, Ting Guo, and Eartha Liu (Abstract)
Vero suspension cells as host to produce oncolytic Measles virus, Aline Zimmer, Dustin Eckhardt, Denise Salzig, Jan Philip Klee, Sascia Neumann, and Andrew Salazar (Abstract)
Targeting glycolytic regulator PFKFB3 reduces excess CHO cell growth removing the bleed stream improving sustainability of the continuous bioprocess, William Smith, Leon Pybus, John Raven, and Alan Dickson (Abstract)
Online monitoring of the respiration activity in 96-deep-well microtiter plate CHO cultures streamlines kill curve experiment, Anne Neuß, Tibor Anderlei, Jochen Büchs, and Jørgen Barsett Magnus (Abstract)
A precise and sustainable doxycycline-inducible cell line development platform for reliable mammalian cell engineering with gain-of-function mutations, Jae Seong Lee, Sung Wook Shin, Jiwon Kim, and Honggi Min (Abstract)
Optimizing transfection condition to improve bispecific antibody expression in CHO cells, Qingxiang Wei, Shu Wang, Leibin Wang, Chenxing Zheng, and Oscar Lara-Velasco (Abstract)
Accelerated intracellular antibody transport by Sar1A overexpression toward developing high-producing CHO cell lines, Yu Tsunoda, Noriko Yamano-Adachi, and Takeshi Omasa (Abstract and Presentation)
Metabolic cytometry for in-line process analysis, Kanika Goel, Jesung Park, Youbo Zhao, Nareg Ohannesian, Richard Marx, and Seongkyu Yoon (Abstract and Presentation)
Improving copper concentration to enhance the robustness of cell culture production processes, Toshiaki Shimoyama, Akihiko . Ban, Hiroki Inoue, Takeshi Takahashi, Yasuharu Shinoda, Saori Suzuki, and Wakana Suzuki (Abstract)
Development of an automated high throughput transfection, selection and screening process for generation of stable pools expressing BsAb’s based on the common light chain and the knob-into-hole technology, Simone Oldenburg, Nadja Pedersen, Morten Dræby Sørensen, Martin Alsted Bendz, and Simone Oldenburg (Abstract and Presentation)
SiMPl-GS and SiMPl-DHFR: advancing cell line development via synthetic selection markers for next-generation biopharmaceutical production, Gyun Min Lee, Chansik Yoon, Eun-Ji Lee, Yujin Kim, Siyun Joung, Dongil Kim, Heungchae Jung, and Yeon-Gu Kim (Abstract and Presentation)
Production of oncolytic Newcastle disease virus in EB66 cells: Characterization of parameters for process intensification, Lennart Jacobtorweihe, Frauke Braatz, Brice Madeline, Arnaud Léon, Yvonne Genzel, and Udo Reichl (Abstract and Presentation)
Identifying manufacturing facilities for concentrated fed batch, Brian Kwan, Dante . Romanini, and Nitya Jacob (Abstract)
Development of a process toolbox to modulate glycosylation of therapeutic proteins, Linda Hoshan, Jared Dopp, Michael Borys, Eric Hodgman, Greg Keil, Ping Xu, Peter Onyskiw, Grace Yao, Rachel Egan, Dane Kazem, Diana Chereches, and Anurag Khetan (Abstract)
Evaluation of advance bioreactor scale-down models for an intensified cell culture perfusion platform process, Jennifer Nguyen, Alaina Anand, Wenge Wang, Krystal Burbank, Matt Gagnon, and Aravindan Rajendran (Abstract)
Establishment of novel CHO-MK cells and optimal culture strategy for enhancement of therapeutic protein production, Erike Sukowati, Junshin Iwabuchi, Ryotaro Fujimoto, Takayuki Horiuchi, and Michi Kubota (Abstract and Presentation)
Highly efficient transposases for single- and multi-target integration of transgenes in mammalian cells, Nicolas Marx, Zerina Arnautalic, and Nicole Borth (Abstract)
Scalable stem cell-based platform to produce tissue specific extracellular vesicles, Rachel Moen, Gabriella Faircloth, Jamey Young, and Ethan Lippmann (Abstract)
Feedback regulated expression of therapeutic proteins for enhanced Biomanufacturing, Carlos Llanos and Laura Segatori (Abstract)
End-to-end process models as life cycle companions, Meena George, Scott Tran, Shengchun Guo, Xinlei Pan, Frederick Rudolph, Andrea Coleman, Marlene Castro-Melchor, and Thomas Zahel (Abstract)
Establishing an intensified fed-batch model in the Ambr250 high throughput system, Andres Martinez, Paul Nguyen, and Amy Vandergriff (Abstract)
Integrated process development for early-stage bispecific antibody, Jun Luo, Weize Yuan, Chenlu He, Chengcheng Liu, Tianyi Gu, Shujing Sun, and Quan Feng (Abstract)
The modern cell line development toolbox: Integrating machine learning and genome editing into automated CLD workflow, Yizhou Zhou, Allen Alcivar, Duncan McVey, Joao Cunha, Maram Essawy, Chaojie Wang, Arathi Krishnakumar, and Anurag Khetan (Abstract)
Validation of the automated Beacon Optofluidic System for ensuring monoclonality of production cell lines, Tina Hummelshøj, Gert Bolt, and Christina Andersen (Abstract)
Cross-species application of bacterial GS selection markers to enhance CHO cell line performance via selection stringency modulation, Simon Fischer, Daniel Heinzelmann, Nicholas Michelarakis, Joschka Bauer, enjamin Lindner, Patrick Schulz, Benjamin Renner, Moritz Schmidt, and Franziska Reuss (Abstract)
Biomanufacturing and lipidomics analysis of extracellular vesicles secreted by human blood vessel organoids in a vertical wheel bioreactor, Justice Ene, Chang Liu, and Yan Li (Abstract)
13C Metabolic flux analysis of HEK producer cell lines to enhance lentiviral vector production, Jamey Young, Paulena Lieske, Kevin D. Smith, Omar El-Ghouch, Sara Weidenbach, and Jamey D. Young (Abstract)
Phase-specific mitochondrial metabolism regulation of CHO cells for enhanced monoclonal antibody yield, Kevin Ruiz-Marquez, Lauren Kraft, Michael J. Betenbaugh, and Jamey D. Young (Abstract and Presentation)
Developing a low-cost prophylactic monoclonal antibody for malaria, Chun Chen, Yinges Yigzaw, and Hong Liu (Abstract)
Hybrid modeling approach for cell metabolism and lactate accumulation in biomanufacturing, Kevin Clark and Jason Chen (Abstract)
Lessons learned in network advancement of process analytical technologies, Andrew Maloney, Juan Parra, Jakob Morris, Spencer Holt, Kyle McElearney, Paul Gramlich, and Glen Bolton (Abstract)
Development of a universal Raman spectroscopy model for real-time monitoring of cell culture production parameters, Laetitia Macon, Paul DAVID-OLIVIER, and Souaifi-Amara HAJER (Abstract)
Purine precursor supplementation increases productivity of antibody-expressing CHO cells by elevating intracellular ATP level, Jong Youn Baik and Joo Hee Bae (Abstract)
Using a multi-omics approach to identify targets for improved cell metabolism in fed-batch and perfusion cultures, Jimmy Kirsch, Brian McConnell, Xiaokun Dong, Diandra Martinez Cano, Kyle McElearney, Angel Santiago-Lopez, Pomaikaimaikalani Yamaguchi, and Kyongbum Lee (Abstract)
Mitigating commercialization challenges for in-licensed programs, Arpan Bandyopadhyay, Esther Lim, Marcella Yu, Huong Le, Laura Matos, Niki Fujimoto, Jimmy Pitingolo, Hiro Ichikawa, Nitya s Jacob, Emilia Galka, and Ronald De Marco (Abstract)
Accelerating monoclonal antibody titer prediction and cell culture medium optimization via nonparametric regression empowered transfer learning, Liang Zhao, Min Chen, Li Fan, and Ziwei Zhou (Abstract)
Advancing adeno-associated virus vector manufacturing: a comparative study of transient transfection and stable producer cell lines, Alana C. Szkodny, Gaurav Chauhan, Pedro A. Moura, Stefan Seeber, Nicole Schueler, Andreas Schaubmar, Hendrik Knoetgen, Julia E. Hölper, Ines do Carmo Gil Goncalves, Michael Mühle, and Michelle Hussong (Abstract)
High demand, low titer, no change of quality accepted -The ancient challenge in a modern world- A case study, Julia Marini, Ines Rüddel, Oliver Bähner, Markus Emmler, and Britta Dalenbrook-Heil (Abstract)
Metabolic analysis of newly established Chinese hamster-derived cell lines, CHL-YN and CHO-MK, Takeshi Omasa, Puriwat SUKWATTANANIPAAT, Sayaka NAGAE, and Noriko YAMANO-ADACHI (Abstract and Presentation)
Understanding transcriptional variance in CHO cells: A quantitative meta-analysis, Markus Riedl, Nicolas Marx, and Nicole Borth (Abstract and Presentation)
Reprogramming lipid metabolism in Chinese hamster ovary cells for enhanced recombinant biotherapeutic protein production, Ankita Mishra, James Budge, and C Mark Smales (Abstract)
Improvement in productivity of protein biologics in recombinant CHO cells created with transposon technology, Tiffany Mclamarrah, Jason Vitko, Jack Scarcelli, Thomas King, and Sara Maimouni (Abstract)
Integrating mechanistic modelling with process measurements for cross-cell line state and parameter estimation in CHO cell culture, Luxi Yu, Antonio del Río Chanona, and Cleo Kontoravdi (Abstract)
Strategic vector design to improve asymmetric bispecific antibody assembly and purity with high expression levels in the GOCHO™ platform, Sheffali Dash, Suet Yan Kwan, Seungjo Park, and Noah Weyrick (Abstract and Presentation)
Characterization of extrinsic chromophores and their impact on therapeutic protein, Dominique Monteil, Rodrick Garcia, Neeraj Pakala, Zherylynn Vinyard, and Adam Catherman (Abstract)
A scale-down semi-perfusion platform coupled with dynamic metabolic flux analysis to inform continuous perfusion process design, Nikola Malinov and Shivam Barodiya (Abstract)
Engineering of the tRNA landscape in CHO cells for enhanced recombinant biotherapeutic protein production, Kishor Elango, Sathishkumar Kurusamy, and C. Mark Smales (Abstract)
Data-driven optimization of perfusion processes to reduce throughput volumes and increase productivity, Srikanth Rapala and Sarah W. Harcum (Abstract)
Upstream process parameter optimization for product quality: A comparative study of statistical and probabilistic design space identification, Syazana Mohamad Pauzi, Ranjith Chiplunkar, and Cleo Kontoravdi (Abstract)
Development of a novel transposase for cell line development, Nikolas Zeh, Mike Leonard, Kevin D. Smith, Brendan Eder, and Scott Estes (Abstract)
Process improvements to the CLD workflow lead to higher producing clones, Christopher Tsiros, Michelle Sabourin, Zhou Jiang, Sheffali Dash, and Seungjo Park (Abstract)
Detachable CD3/CD28 magnetic beads: A convenient and flexible upstream solution in T cell therapy manufacturing, Andrew Campbell, Michael . Bruno, Anna-Barbara Hachmann, Heidi Vebø, Evan Zynda, Hany Meås, Maxi-Lu Bӧschen, Neda Moharrami, Ingrid Moen, Jenny Rasmussen, Marta Tellez Gabriel, and Kyle Zastrow (Abstract)
Multi-level characterization of NISTCHO: Temperature shift affects phenotype and critical quality attributes, Larissa Hofer, Christian G. Huber, Thomas Rauter, Veronika Schäpertöns, Wolfgang Esser-Skala, Nikolaus Fortelny, and Thomas Berger (Abstract and Presentation)
Bioprocessing of stem-cell derived allogeneic cell therapy products - enabling large-scale cell harvesting using single-use centrifugation, Mats Akesson, Chee Keong Kwok, Arvind Pradip, and Michael Wahlers (Abstract)