April 27 - May 2, 2025
Tucson, AZ
| Chairs: | A Khetan, BMS |
|---|---|
| Marcella Yu, Gilead | |
| Mike Betenbaugh, Johns Hopkins University |
25 conference program - Cell Culture Engineering XIX, Michael Betenbaugh, Anurag Khetan, and Marcella Yu (Article)
Developing Programmable Medicines, Avak Kahvejian (Abstract)
Development of an optimized FIH process to accelerate a multi-modality drug discovery pipeline, Ravali Raju, Keshab Rijal, Natalia Gomez, Amlan Das, Kristi Daris, Varada Dandekar, Juliane Glaser, and Landon Mott (Abstract)
Unlocking productivity in an upstream continuous process by combining media concentrates and multi-phase feeding, Nuno Carinhas, Mengyao Li, David Garcia, Diana Lupea, Adriano Del Priore, Werner Nelson, and Sylvius Willerich (Abstract and Presentation)
Model-based scale-up of CHO cell culture processes for accelerated technology transfer, Emmanuel Anane, Budi Juliman, and Rejhan Klica (Abstract)
Lessons learned from scaling and improving tech transfer and the manufacturing process: a history of 1000L to 18,000L Cell Culture mAb production, Sarwat Khattak (Abstract)
Global access needs innovations for producing affordable monoclonal antibodies and cell culture-based vaccines with easy to use delivery technologies, Vijay Yabannavar (Abstract and Presentation)
History Repeats: Leveraging process intensification strategies for monoclonal antibodies to quadruple lentiviral vector production, Charles Hill, Xiaojun Liu, and Chris Brown (Abstract)
State of the ARC; Manufacturability of an antibody-siRNA conjugate, Ronan Michael Kelly and Chris C. Frye (Abstract)
Pilot-scale generation of antibody retargeted AAV viral vectors using a hexad transfection, Debby Liu, Zhe Zhang, Hanne Bak, Garima Thakur, Andrew Tustian, Louis Coplan, and Sheldon Mink (Abstract)
Glucagon-like peptide-1: Its development and role in diabetes and obesity, John Mayer (Abstract)
Optimization of multispecific antibody production: the impact of vector topology, ratio, and selection strategies for improved productivity and quality, José Escandell, Sara . Maimouni, Catarina Melo, Mariana Antunes, Jose Escandell, Michael . Hoffman, Patrícia Gomes-Alves, and Jack . Scarcelli (Abstract)
Antibody-Drug Conjugates (ADCs): Design, Developing Trends and Future Prospects, John M. Lambert (Abstract)
Professor Wei-Shou’s Career: Educating generations of leaders & advancing the biopharmaceutical industry, Nitya Jacob (Abstract)
When the means becomes the end: CCE for cell therapy products, Susan Abu-Absi (Abstract)
Integrative systems approach to cell line design: From bistability to metabolic byproduct reduction to amino acid prototrophy, Bhanu Chandra Mulukutla and Chris McCready (Abstract and Presentation)
Perspectives from the early years, and an inspiration for a lifetime, Jamie Piret (Abstract)
Wei-Shou Hu and industrial cell culture, John Aunins (Abstract)
Transfection 101: Tips that lead to improvements in upstream processing for AAV manufacturing, Sandy Tseng (Abstract)
Advancing multispecific molecule manufacturing through optimized vector design and CHO cell line development, Stephen Jaffé and Arina Alfred (Abstract)
Enabling expedited biologics process development and robust manufacturing by Raman-based PAT, Hang Zhou, Weichang Zhou, Zhe Lang, and Mingyue Fang (Abstract)
Doubling recombinant protein productivity in CHO cells and altering glycosylation profiles by modulating underexplored autophagy and cellular growth mechanisms, Sri Madabhushi, Ansuman Sahoo, Tomas Kasza, Tomali Chakravarty, Taku Tsukidate, and Xuanwen Li (Abstract)
Application of data-driven upstream levers and hybrid models to control Afucosylation at desired levels in CHO cell cultures, Khandaker Siddiquee (Abstract)
Interplay between amino acid depletions, Sequence variants and other product quality attributes in a bispecific mAb: A case study, Nancy Guillen, Nantiya Moonwong Abraham, Jason Chen, Pushpraj Rana, Nicole Larmore, Fang Qian, and Adam Evans (Abstract)
Model-driven genetic design and bioprocess optimization across modalities, Imroz Ghangas, Brianna Jayanthi, Alec Nielsen, Will Johnson, Kevin Smith, Adam Carcella, and Nikolas Zeh (Abstract)
Stories of Growth in Model-Based Process Development, Monitoring, and Control, Yu Luo (Abstract)
Galileo revisited: The end of the genocentric view of the cell, Nicole Borth, Federico DeMarco, Ivy-Rose Sebastian, Antonino Napoleone, Alexander Molin, Mohammed Hussein, and Nicolas Marx (Abstract)
Speed: Accelerating antibody drug discovery and development through advances in transient and stable gene expression technologies, Gavin Barnard (Abstract)
Systems biology, cell line and protein engineering for increased AAV production, Johan Rockberg, Maximilian Karlander, Alice Karlsson, Magdalena Malm, and Niklas Thalen (Abstract)
Engineering mammalian cell growth dynamics for biomanufacturing, Alan Dickson and Mauro Torres (Abstract)
From insect to impact: Discovery of a novel chimeric transposase system to advance mammalian cell factories, Simon Fischer, Daniel Heinzelmann, Benjamin Renner, Patrick Schulz, Moritz Schmidt, Franziska Reuss, and Nikolas Zeh (Abstract)
Leveraging generative artificial intelligence for optimized biologic design and production, Adam Root (Abstract)
Hybrid modeling for in silico optimization and supervisory control of Perfusion cell culture processes, Gerben Zijlstra and Chris McReady (Abstract)
Data- and knowledge-driven modeling of cell culture processes, Qi Zhang, Yen-An Lu, Prahalad Srinivasan, and Wei-Shou Hu (Abstract)
Autonomous process development for cell culture processes, Nandita Vishwanathan, Thomas Vuillemin, Jean-Marc Bielser, Chethana J Gadiyar, Claudio Mueller, Michael Sokolov, and Nico Cruz (Abstract and Presentation)
Quantifying metabolism of CHO cells using comprehensive stable- isotope tracing, Maciek Antoniewicz (Abstract)
Computational bioprocess: The role of robust virtual presentations in bioprocess, Zheng Huang and Yossi Quint (Abstract)
Cultivated meat: A new frontier for hungry cell culture engineers, Robert D. Kiss (Abstract and Presentation)
Improving CAR T cell potency through process control, Marta Costa, Margarida Costa, Constança Costa, Leonor Matos, Hélio Tomás, Paula Marques Alves, Margarida Serra, Juan R. Rodriguez-Madoz, Patricia Jauregui, Saray Rodriguez-Diaz, and Felipe Prosper (Abstract)
Implementation of 3D bioreactors to deliver expansion and patient access potential of allogeneic iPSC-derived cell therapies, Chen-Yuan Kao, Tom Donnelly, Christopher Deborde, Kyle Smith, Jason A. Mills, Margaret Brennan, and Bruno Marques (Abstract)
Scaling mRNA medicines: Unlocking the power of Moderna’s platform across vaccines and therapeutics, Shyam Subramanian, (Abstract)
Driving Innovations to Secure the Future of Cell Culture Bioprocessing, Weichang Zhou (Abstract and Presentation)
Engineering the future of AAVs - Driving cost-efficiency, market access, and regulatory success in gene therapy, Sven Markert (Abstract)
Advancing AAV manufacturability toolbox for early phase programs and beyond, Ayda Mayer, Amanda Zhang, Craig Malzahn, and Bonnie Wu (Abstract)
A novel, site-specific N-linked glycosylation model provides mechanistic insights into the process-condition dependent distinct Fab & Fc glycosylation of an IgG produced in CHO VRC01 cells, Eleftherios Papoutsakis, Jayanth Venkatarama Reddy, Marianthi Ierapetritou, Thomas Leibiger, and Sumit Kumar Singh (Abstract)
Removing rhabdovirus (RV) from cell culture process to enable RV-negative production of rAAV, Luhong He, Christopher Hatfield, Hunter Balduf, and Michael Lloyd (Abstract)
Addressing nutritional and sensory qualities of cultivated meat with COGS and regulatory considerations, Jayanthi Lakkyreddy (Abstract)
Production of a fusogenic oncolytic virus: Establishment of a scalable integrated production process, Sven Gobel, Rita Fernandes, Cristina Peixoto, Jennifer Altomonte, Yvonne Genzel, and Udo Reichl (Abstract)
Next-Gen "Super" CHO host development: Metabolically engineered host with cysteine/tyrosine prototrophy and negligible BCAA metabolic byproduct formation, Laura Greenfield, Mariah Brantley, Laura Waller, and Bhanu Chandra Mulukutla (Abstract and Presentation)
Setting recombinant AAV manufacturing for success with smart process design and engineering, Nic Preyat (Abstract)
Developing an improved cell line development platform for the production of complex multi-specific antibodies, Jie Zhu (Abstract)
Developing Iinducible stable cell lines for rAAV production, Seongkyu Yoon (Abstract)
Enhanced ER protein processing gene expression increases rAAV yield and full capsid ratio in HEK293 cells, Yongdan Wang, Qiang Fu, Yongdan Wang, Jiansong Qin, Dongming Xie, and David McNally (Abstract)
Immunocompetent human 3D liver model to accelerate AAV-based ATMP development, Paula Alves, Catarina Brito, Francisca Arez, Isabella Ramella Gal, Giacomo Domenici, Sofia Fernandes, Inês Correia, Gabriela Silva, Nádia Duarte, Catarina Freitas, A S Coroadinha, Udo Maier, and Francois du Plessis (Abstract)
The use of mixture design can improve 109-Fold rAAV titers by plasmid ratio optimization, Jesús Lavado García, Konstantina Tzimou, David Catalán-Tatjer, and Lars K. Nielsen (Abstract)
Assessing the impact of transient dissolved oxygen excursions on process performance and product quality, Cathleen Schiraldi, Patrick Faughnan, Pamela Wright, Daniel Norton, Andrew Lutringer, Katherine Resch, and Christopher Sevinsky (Abstract)
Without a trace: Identification, characterization and stable host cell engineering of CHO hydrolases to tackle the polysorbate degradation challenge, Linus Weiss, Melanie Maier, Simon Fischer, Daniel Lakatos, Nikolas Zeh, and Kerstin Otte (Abstract)
Streamlining cell line development: A case study comparing random integration and transposon system for bispecific antibody, Yashas Rajendra and Milly Milton (Abstract)
Machine-learning assisted clone selection yields improved clone titers in intensified cell culture processes, Brandon Downey, John Schmitt, Søren Flygenring-Basset, Julia Armstrong, Shannon Hendrick, Christian Siltanen, and Nicolas Wolnick (Abstract)
Innovative horizons: Perfusion-driven process intensification for HEK293 cells in cell and gene therapy, Vera Ortseifen, Pia Brinkert, Niklas Kraemer, Tim Steffens, Sandra Klausing, Dennis Karthaus, Anica Schmidt, Kathrin Teschner, and Vera Ortseifen (Abstract)
Lifecycle management of a commercial monoclonal antibody platform: understanding the impact of raw material variability and its impact on supply resilience, Cillian McCabe, Will Pierce, and Deirdre Buckley (Abstract)
Tuning lactate metabolism with Raman enabled automated lactate control, Venkata Gayatri Dhara, Jennifer Quaglia-Motta, Guogang Dong, and Edwin Ming-Hong Shen (Abstract)
Effect of AAV and AdV gene expression on the transcriptome and proteome of CHO cells, Konstantina Tzimou, Lars Keld Nielsen, and Jesús Lavado-García (Abstract)
NISTCHO: Use of open access living reference material for enhancing tech transfer efficiency and product understanding, Hussain Dahodwala, John P. Marino, and Zvi Kelman (Abstract)
“Organized stress” for robust scale-up of intensified process with non-perfusion seed bioreactor, Bassem Yahia, Antoine Piednoir, and Thomas Dahomais (Abstract and Presentation)
Multiplex genome editing to eliminate the Warburg effect and attenuate ammonia production, Hooman Hefzi, Karen Julie la Cour Karottki, Bjørn Voldborg, and Nathan Lewis (Abstract)
Raman spectroscopy as an enabling technology for advanced control strategies for upstream bioprocessing, Matthew Zustiak; Juan Villa, Ph.D; Lin Zhang; and Nimesh Khadka (Abstract)
Epigenomics: A key player in the omics revolution driving digital twins and in-silico bioprocess optimization, Karlheinz Landauer, Daniel Luescher, Maxwell Pryce, and Asier Galarza (Abstract and Presentation)
Advanced manufacturing controls using process analytical technologies (PAT) to enable robust and productive drug substance processes, Kyle McElearney, Sneha Suman, Glen Bolton, Andy Maloney, Stephanie Kim, Tony Wang, Namratha Subhash, Harrison Beamer, Jakob Morris, Spencer Holt, Michaela Murr, and Ronak Gudhka (Abstract)
Late-stage development and upstream process characterization of UX701, an AAV gene therapy for Wilson Disease, Jan Panteli, Jun Li, Patrick Hossler, James Warren, Benjamin Hanson, and Tiego Bezerra (Abstract)
N-1 perfusion platform technology development, implementation, and business case analysis in commercial manufacturing, Jianlin Xu, Jonathan Forstrom, Elien Van Sinay, Yang Wu, Henry Lin, Charles Budde, and Agathe Drape (Abstract)
Mitigating scale-up challenges: The impact of feed media filtration and storage on CHO cell culture performance at 2000 L scale, Abhinav Jain, Yashas Rajendra, and Vikram Sisodiya (Abstract)
Optimization of an upstream process improves production and preserves product quality through a multifaceted and intensified approach, Anna Peterson (Abstract)
Developing a digital twin for In Silico medium development, Amy Johnson; Daniel Eckstein; Hanne Bak,; Shawn Lawrence; and Sarah Mbiki (Abstract)
Next generation of genetically engineered stable producer cell lines to overcome viral vector manufacturing challenges, Robert Whitfield, Tobias Bonifert, and Helmut Kewes (Abstract)
Supertransfection vs. co-transfection: An evaluation of two orthogonal transposases for improving protein expression in CHO cells, Melina Lenser, Hanh (Julie) Ngo, Yashas Rajendra, and Lily Sarrafha (Abstract)
Enhancing bioreactor performance predictions by integrating process conditions into dynamic metabolic flux analysis models, Marianthi Ierapetritou, Jayanth Venkatarama Reddy, Nikola Malinov, and Eleftherios Papoutsakis (Abstract)
Developing a next-gen process for BCAT1 KO CHO host to enable robust lactate shift and higher productivities, Mariah Brantley, Jin Guo, Wenge Wang, Bhanu Chandra Mulukutla, and Maddie Andres (Abstract and Presentation)
Next generation approaches to genetic characterization of Chinese hamster ovary cell lines using next generation sequencing, Sofie O'Brien, Colin Anderson, Mark Gerber, Jill Ward, and Wei Wei (Abstract)
New transposase integration in SUREtechnology PlatformTM takes high-performance clones to the next-level, Juliette Trepreau, Audrey Dubost, Emanuel Schmidt-Siegert, Alexia Borgeat, Louis Dumas, and Quentin Aeberli (Abstract)
Derisking tech transfer using process understanding and functional bioassay, Thu Phan, Dante Dorian, Nikki Li, Riya Patel, Gisel Lopez, Son Le, Emilia Galka, Arpan Bandyopadhyay, Jenny Wang, Huong Le, Nitya Jacob, and Marcella Yu (Abstract)
Acceleration of knowledge-based process development through application of hybrid modeling, Karna Shah, Brendan McGuire, Nicholas Abu-Absi, Delia Lyons, Lauren Guertin, Tan Dao, Clayton Wandishin, and Anisur Rahman (Abstract)
Autophagy and Akt-stimulated cellular proliferation synergistically improve antibody production in CHO cells, Anne Robinson, Leran Mao, and Sarah Sonbati (Abstract and Presentation)
Integrative Analysis of Metabolomics, Lipidomics, and Process Outputs in CHO Cells: Unveiling Correlation Patterns, Sandeep Ranpura; Maria Sani; Gheorghe Alexandra-Gabriela; Hatwan Galali; Deniz Demirhan,; and Sabrina Liberatori (Abstract)
Integration of triple gene vectors for improved expression and product quality of multispecific molecules, Lauren Kraft and Thomas Kelly (Abstract)
Transposon-based gene integration strategies for seamless cell line development workflow (Protein Biologics & Gene Therapy), Saurabh Sen, Sarah Child, Rebecca Jeppson, Mahtab Dastpak, Christine DeMaria, Henry Lin, and Elizabeth Masterjohn (Abstract)
Revealing scale down model challenges of biopharmaceutical upstream processes, Michael Loeffler, Timm Keil, Thomas Wucherpfennig, Jochen Schaub, and Jürgen Fitschen (Abstract)
Investigating the metabolic load of monoclonal antibody production conveyed to an inducible CHO cell line using a transfer-rate online monitoring system, Juan Sebastian Reyes, Lucas Lemire, Olivier Henry, Yves Durocher, and Phuong Lan Pham (Abstract and Presentation)
Elucidating mechanisms for hollow fiber filter fouling, Samik Das, Emma Eifert, Ken Lee, Maxine Annoh, and Jon Coffman (Abstract)
Designing cell factories for difficult to express proteins, Verónica S. Martínez, Zhuoqi Xiao, Irene Bharali, Marianne Gillard, Mary Otto, Jessica Cuthber, Min Zhang, Adrian Mazzone, Trent Munro, Lars K. Nielsen, Kym Baker, Esteban Marcellin, and Verónica S . Martínez (Abstract and Presentation)
Developing a high-productivity and robust ultra-intensified WuXiUI™ bioprocess strategy empowered by pat and in-silico process modeling, Jun Tian, Le Yu, Weijia Cao, Hao Tang, Shaoxun Xiang, and Jinliang Zhang (Abstract)
Integrating long-read sequencing and multiomics for precision cell line engineering, Mounika Boddireddy, Daniel K. Fabian, Pavle Vrljicak, Bernadette Sweeney, Alessandro Di Cara, and Arina Alfred (Abstract)
Machine learning-based hybrid model enables accelerating the upstream process development in CHO cells, Michael Borys, Michael Borys, and Anurag Khetan (Abstract)
Cell engineering to optimize viral vector production, Kerstin Otte, Madina Burkhart, Bettina Finkbeiner, Jona Röscheise, Andreas Schulze, Markus Hörer, Parameswari Govindarajan, and Holger Laux (Abstract)
Optimized gene configuration design for enhanced production of standard and complex antibodies in targeted integration CHO cell lines, Zion Lee, Eric . Nshimyumukiza, Gavin Barnard, Amy Shen, and Jun Wan (Abstract)
A framework approach for application of a chemometric-based screening method to predict raw material variability in bioprocesses, Geraldine Kelly, Sarah Henry, Matthew D. Osborne, and Alan G. Ryder (Abstract)
Innovative process intensification for adenoviral vector production: A path to faster and higher product yields, Alena Roßkamp, Kim B. Kuchemüller, Alexander D. Douglas, Chris . McCready, and Julia Niemann (Abstract)
Intensified perfusion culture enabling high productivity, high product quality, and low manufacturing cost, Weichang Zhou, Hang Zhou, Mingyue Fang, and Xiang Zheng (Abstract)
Simultaneous enhancement in AAV productivity and particle quality in batch and perfusion cultures of producer cell lines through systems approach, Madhuresh Sumit, Nipun Goel, Daniel Furey, Olivia Tsang, Rachel Sun, Yingchao Nie, Karen Vincent, Jeong Lee, and Bruno Figueroa (Abstract)
Development of a novel mechanistic model for describing the growth and infection / transfection of HEK cells, Kim B. Kuchemüller, Alena Roßkamp, Alexander D. Douglas, Julia Niemann, and Chris . McCready (Abstract)